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Showing: 1 - 20 of 41 results

  2. pGLU_63

    Plasmid
    #225766
    Purpose
    This is the native promoter from the tetL tetracycline resistance cassette.
    Depositor
    Nili Ostrov
    Insert
    PtetL_[abr]
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    N/A
    Promoter
    Available since
    Oct. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    225766-plasmid-map-sequence-id-447306

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  5. pCas9

    Plasmid
    #167547
    Purpose
    Used for CRISPR-Cas9 mediated recomineering in Enterococcus. Can clone desired gRNA using BsaI digestion.
    Depositor
    Howard Hang
    Article
    Chen et al Appl Environ Microbiol. 2021 Jul 7:AEM00
    Inserts
    chloramphenicol acetyl transferase
    pUC19 origin of replication
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    167547-plasmid-map-sequence-id-332733

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  6. pRecT_2

    Plasmid
    #167546
    Purpose
    Encodes an IPTG inducible recT from E. faecium Com15 used to ectopically express recT in Enterococcus for recombineering.
    Depositor
    Howard Hang
    Article
    Chen et al Appl Environ Microbiol. 2021 Jul 7:AEM00
    Insert
    E. faecium Com15 recT under an IPTG inducible promoter, with lacI
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    IPTG
    Available since
    June 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    167546-plasmid-map-sequence-id-332204

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  7. pKS1469

    Plasmid
    #218588
    Purpose
    Express peroxisomal MvaSp and MvaEp to convert AcCoA to mevalonate
    Depositor
    John Dueber
    Insert
    MvaS
    Use
    Tags
    ePTS1
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Oct. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    218588-plasmid-map-sequence-id-431884

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  12. pSMART-Mev1

    Plasmid
    #65815
    Purpose
    NADPH Dependent Mevalonic Acid Production Construct, Low Phosphate Dependent E. coli Expression
    Depositor
    Michael Lynch
    Inserts
    mvaE: bifunctional acetoacetyl-CoA thiolase, and NADPH dependent HMG-CoA reductase
    mvaS: hydroxymethylglutaryl-CoA synthase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    insulated mipAp from E. coli and insulated waaHp…
    Available since
    Sept. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    65815-plasmid-map-sequence-id-111947

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  18. pUCsRNAP

    Plasmid
    #182746
    Purpose
    Swapping in small RNA promoter, 23-bp spacer sequence, & 19-bp repeats into pJC005 plasmids
    Depositor
    James Collins
    Article
    Chua et al Microbiol Spectr. 2022 Feb 2:e0242721. d
    Insert
    small RNA promoter, a 23-bp spacer sequence, & 19-bp repeats
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    182746-plasmid-map-sequence-id-358079

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Showing: 1 - 20 of 41 results