pET-28a(+)-HMGS
(Plasmid
#108945)
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Purposeheterologous expression of HMGS of E. faecalis in E. coli
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 108945 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET-28a(+)
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature30°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHMG-CoA synthase (HMGS)
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SpeciesEnterococcus faecalis
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MutationA110G
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Tag
/ Fusion Protein
- His6-Tag (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
codon-optimized synthetic gene for expression in E. coli
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET-28a(+)-HMGS was a gift from Frank Schulz (Addgene plasmid # 108945 ; http://n2t.net/addgene:108945 ; RRID:Addgene_108945) -
For your References section:
An in vitro Biosynthesis of Sesquiterpenes starting from Acetic Acid. Schulz F, Dirkmann M, Nowack J. Chembiochem. 2018 Aug 7. doi: 10.1002/cbic.201800128. 10.1002/cbic.201800128 PubMed 30085399