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Addgene

pUCsRNAP
(Plasmid #182746)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 182746 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pUCsRNAP
  • Backbone size w/o insert (bp) 2696
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    small RNA promoter, a 23-bp spacer sequence, & 19-bp repeats
  • gRNA/shRNA sequence
    ATAATCTTTAATTTGAAAAGATTTAAGGCTTATTTAAATAAAAAATATGAGGGAAGAATTGATATAAATTTAATTTTGTTATTGTATTATGGTATGTATGGAATAAATTTAACATAAAGACAGTAATAATGTTCTTGAATTTAGACTTTTTATGTGTTATCATTAACAAGTATCAAAAATGACATTTAATAAATTAATAATAATTTTAAAAATATATTTTTGATAAAAGCAATGATTAACATGGTTTGACGTCTGAGAAGAGACGATTTTCTCAATAGGAGAAATTAAGGTGCAAacccttatcattccaccaTAATTTCTACTCTTGTAGATtcctcaaagagcatatggatatgAATTTCTACTCTTGTAGAT

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUCsRNAP was a gift from James Collins (Addgene plasmid # 182746 ; http://n2t.net/addgene:182746 ; RRID:Addgene_182746)
  • For your References section:

    Rapid, Efficient, and Cost-Effective Gene Editing of Enterococcus faecium with CRISPR-Cas12a. Chua MJ, Collins J. Microbiol Spectr. 2022 Feb 2:e0242721. doi: 10.1128/spectrum.02427-21. 10.1128/spectrum.02427-21 PubMed 35107356