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Showing: 1 - 20 of 894 results

  2. PX385 Streptococcus pasteurianus Cas9

    Plasmid
    #68322
    Purpose
    Streptococcus pasteurianus Cas9
    Depositor
    Feng Zhang
    Insert
    SpaCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    68322-plasmid-map-sequence-id-119537

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  5. pX330-Flag-eSpCas9-D10A

    Plasmid
    #80449
    Purpose
    enhanced SpCas9 mammalian expression vector
    Depositor
    Ervin Welker
    Insert
    ehSpCas9_D10A
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Available since
    Aug. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    80449-plasmid-map-sequence-id-157945

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  6. pX330-Flag-eSpCas9-H840A

    Plasmid
    #80454
    Purpose
    enhanced SpCas9 mammalian expression vector
    Depositor
    Ervin Welker
    Insert
    ehSpCas9_H840A
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Available since
    Aug. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    80454-plasmid-map-sequence-id-151472

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  7. pX330-Flag-HFSpCas9-H840A

    Plasmid
    #80455
    Purpose
    high fidelity SpCas9 mammalian expression vector
    Depositor
    Ervin Welker
    Insert
    HFSpCas9_H840A
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Available since
    Sept. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    80455-plasmid-map-sequence-id-151476

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  9. pX330-Flag-HeFSpCas9-H840A

    Plasmid
    #80456
    Purpose
    high fidelity enhanced SpCas9 mammalian expression vector
    Depositor
    Ervin Welker
    Insert
    HeFSpCas9_H840A
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Available since
    Aug. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    80456-plasmid-map-sequence-id-151478

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  11. p3015b

    Plasmid
    #209317
    Purpose
    Expresses a sgRNA template with a protospacer cloning site flanked by BsaI restriction sites for easy insertion of a targeting cassette.
    Depositor
    Thomas Hooven
    Article
    Gopalakrishna et al Commun Biol. 2023 Jun 9;6(1):620. doi: 1
    Insert
    Group B Streptococcus-compatible single guide RNA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Xyl/tet promoter that is constitutively active
    Available since
    July 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    209317-plasmid-map-sequence-id-435309

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  12. XClone_Cas9_KRAB_T2A_BFP

    Plasmid
    #141208
    Purpose
    Tunable and temporal expression control of dCas9
    Depositor
    Rory Johnson
    Insert
    dCas9
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    141208-plasmid-map-sequence-id-308770

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  14. pTUB1-Spy.dCas9-CAT-U6-sgRNA(decoy)

    Plasmid
    #171089
    Purpose
    CRISPR interference. Construct for expressing catalytically inactive Cas9 (dCas9) from Streptococcus pyogenes in Toxoplasma gondii. Suitable for generating stable cell lines.
    Depositor
    Sebastian Lourido
    Article
    Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1
    Inserts
    Decoy sgRNA
    dCas9
    Chloramphenicol acetyltransferase
    Use
    CRISPR; Toxoplasma gondii expression
    Tags
    3X FLAG and Nuclear Localization Signal
    Expression
    Mutation
    D10A, H840A
    Promoter
    TUB1 (Toxoplasma gondii) and U6 (Toxoplasma gondi…
    Available since
    Oct. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    171089-plasmid-map-sequence-id-339962

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  15. XClone-Cas9-Cronus-T2A-BFP

    Plasmid
    #141209
    Purpose
    Tunable and temporal expression control of Cas9
    Depositor
    Rory Johnson
    Insert
    Cas9
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    141209-plasmid-map-sequence-id-299562

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  16. JDS246

    Plasmid
    #43861
    Purpose
    Expresses mammalian codon optimized Cas9 nuclease with C-term 3X FLAG from CMV and T7 promoters
    Depositor
    Keith Joung
    Insert
    mammalian codon-optimized streptococcus pyogenes Cas9 - 3X Flag
    Use
    CRISPR
    Tags
    3X FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    43861-plasmid-map-sequence-id-59200

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  17. pXGFPC-5 Pxyl-dcas9 (Sth3)

    Plasmid
    #133317
    Purpose
    expresses dcas9 (Streptococcus thermophilus #3); repressed with 0.2% glucose, induced with 0.3% xylose; for homologous recombination at the xyl locus in Caulobacter crescentus; tetracycline resistance
    Depositor
    Michael Laub
    Article
    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:
    Insert
    dcas9 (Streptococcus thermophilus #3)
    Use
    Unspecified
    Tags
    Expression
    Mutation
    Promoter
    xylose
    Available since
    Jan. 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    133317-plasmid-map-sequence-id-266835

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  18. pVCERC-5 Pvan-dcas9 (Sth3)

    Plasmid
    #133319
    Purpose
    expresses dcas9 from Streptococcus thermophilus #3; induced with vanillate; for homologous recombination at the vanillate locus in Caulobacter crescentus; tetracycline resistance
    Depositor
    Michael Laub
    Article
    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:
    Insert
    dcas9 (Streptococcus thermophilus #3)
    Use
    Unspecified
    Tags
    Expression
    Mutation
    Promoter
    vanillate
    Available since
    Jan. 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    133319-plasmid-map-sequence-id-266830

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  19. pXGFPC-5 Pxyl-dcas9 (Spa)

    Plasmid
    #133318
    Purpose
    expresses dcas9 (Streptococcus pasteurianus); repressed with 0.2% glucose, induced with 0.3% xylose; for homologous recombination at the xylose locus in Caulobacter crescentus; tetracycline resistance
    Depositor
    Michael Laub
    Article
    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:
    Insert
    dcas9 (Streptococcus pasteurianus)
    Use
    Unspecified
    Tags
    Expression
    Mutation
    Promoter
    xylose
    Available since
    Feb. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    133318-plasmid-map-sequence-id-266833

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  20. pXGFPC-5 Pxyl-dcas9 (Spy)

    Plasmid
    #133316
    Purpose
    expresses dcas9 from Streptococcus pyogenes; repressed with 0.2% glucose, induced with 0.3% xylose; for homologous recombination at the xylose locus in Caulobacter crescentus; tetracycline resistance
    Depositor
    Michael Laub
    Article
    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:
    Insert
    dcas9 (Streptococcus pyogenes)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    xylose
    Available since
    Feb. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    133316-plasmid-map-sequence-id-266825

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Showing: 1 - 20 of 894 results