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Addgene

XClone_Cas9_KRAB_T2A_BFP
(Plasmid #141208)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 141208 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    XLone-GFP
  • Vector type
    Mammalian Expression
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dCas9
  • Alt name
    catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
  • Species
    Streptococcus pyogenes

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

derivatives of Addgene plasmid # 96930

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    XClone_Cas9_KRAB_T2A_BFP was a gift from Rory Johnson (Addgene plasmid # 141208 ; http://n2t.net/addgene:141208 ; RRID:Addgene_141208)