pGEXndoS(E235Q)
(Plasmid
#44656)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 44656 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGEX-5X-3
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Backbone manufacturerGE Healtcare
- Backbone size w/o insert (bp) 4974
- Total vector size (bp) 7862
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namendoS
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Alt nameEndoglycosidase S
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SpeciesStreptococcus pyogenes
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Insert Size (bp)2878
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MutationChanged Glutamic acid 235 to Glutamine
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GenBank IDAF296340
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Entrez GenendoS (a.k.a. SpyM3_1568)
- Promoter tac
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Tag
/ Fusion Protein
- Gst (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer 5' pGEX sequencing primer
- 3′ sequencing primer 3' pGEX sequencing primer (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEXndoS(E235Q) was a gift from Mattias Collin (Addgene plasmid # 44656 ; http://n2t.net/addgene:44656 ; RRID:Addgene_44656) -
For your References section:
EndoS from Streptococcus pyogenes is hydrolyzed by the cysteine proteinase SpeB and requires glutamic acid 235 and tryptophans for IgG glycan-hydrolyzing activity. Allhorn M, Olsen A, Collin M. BMC Microbiol. 2008 . 8():3. 10.1186/1471-2180-8-3 PubMed 18182097