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Showing: 1741 - 1760 of 157695 results

  3. pCryptDel4.8

    Plasmid
    #141293
    Purpose
    Plasmid for curing pMUT2 in one step based on pFREE. Contains RelB antitoxin, as well as gRNA targetting pMUT2 plasmid as well as pCryptDel4.8 itself.
    Depositor
    Neel Joshi
    Article
    Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102
    Inserts
    RelB
    gRNA targetting pMUT2
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Native promoter from pMUT2 relB/relE operon and P…
    Available since
    Jan. 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    141293-plasmid-map-sequence-id-322822

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  6. pLVX-EGFP-C1-centrin-1

    Plasmid
    #73331
    Purpose
    Lentiviral vector encoding centrin-1 with an N-terminal EGFP tag
    Depositor
    Manuel Thery
    Insert
    centrin-1 (CETN1 Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    73331-plasmid-map-sequence-id-139044

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  9. pSR657

    Plasmid
    #65214
    Purpose
    Baculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-2 cap gene in Sf9 insect cells
    Depositor
    Robert Kotin
    Article
    Smith et al Mol Ther. 2009 Nov;17(11):1888-96. doi:
    Inserts
    modified AAV-2 rep78
    modified AAV-2 cap
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    p10 and polyhedrin
    Available since
    June 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    65214-plasmid-map-sequence-id-116341

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  15. N-Terminal Split Cas9 with GyrA intein

    Plasmid
    #58693
    Purpose
    Expresses truncated N-terminal SpCas9 domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with C-Terminal Split Cas9 Gyra Intein for full length SpCas9 production
    Depositor
    Gang Bao
    Article
    Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103
    Insert
    Humanized N-Terminal S. pyogenes Cas9 with GyrA Nsplit Intein
    Use
    AAV and CRISPR
    Tags
    3xFlag, GyrA Nsplit Intein, and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Available since
    July 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    58693-plasmid-map-sequence-id-90684

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  19. PEA1-GFP

    Plasmid
    #171993
    Purpose
    Delivers all prime editing nickase components in a single, GFP selectable plasmid
    Depositor
    Paul Thomas
    Article
    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637
    Insert
    CbH-Cas9(H840A)-RT-T2A-GFP, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV for Cas9, U6 for gRNAs
    Available since
    Aug. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    171993-plasmid-map-sequence-id-340552

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  20. RXFP1-Tango

    Plasmid
    #66492
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    RXFP1 (RXFP1 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 1741 - 1760 of 157695 results