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Next-generation GRAB sensors for monitoring dopaminergic activity in vivo.

Sun F, Zhou J, Dai B, Qian T, Zeng J, Li X, Zhuo Y, Zhang Y, Wang Y, Qian C, Tan K, Feng J, Dong H, Lin D, Cui G, Li Y
Nat Methods. 2020 Nov;17(11):1156-1166. doi: 10.1038/s41592-020-00981-9. Epub 2020 Oct 21. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
140553pAAV-hsyn-GRAB_DA2mExpresses the 2nd generation medium-affinity DA sensor GRAB_DA2m in neurons
140554pAAV-hsyn-GRAB_DA2hExpresses the 2nd generation high-affinity DA sensor GRAB_DA2h in neurons
140555pAAV-hsyn-GRAB_DA-mutExpresses the DA-insensitive control sensor GRAB_DA-mut in neurons
140556pAAV-hsyn-GRAB_rDA1mExpresses the red fluorescent medium-affinity DA sensor GRAB_rDA1m in neurons
140557pAAV-hsyn-GRAB_rDA1hExpresses the red fluorescent high-affinity DA sensor GRAB_rDA1h in neurons
140558pAAV-hsyn-GRAB_rDA-mutExpresses the red fluorescent DA-insensitive control sensor GRAB_rDA-mut in neurons

Antibodies from Article