Addgene

We narrowed to 2,262 results for: PRK

Showing: 1581 - 1600 of 2262 results

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pGEX-4T-1 3xFlag-JNK1a1

Plasmid

#47574

Purpose

Bacterial expression plasmid for GST-3xFlag-tagged JNK1a1

Depositor

Kevin Janes

Article

Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

Insert

JNK1a1 (MAPK8 Human)

Use

Tags

3xFlag and GST

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 28, 2013

Availability

Academic Institutions and Nonprofits only
CALCOCO1-HA

Plasmid

#137766

Purpose

Expression and localization of tagged CALCOCO1

Depositor

Carol Mercer

Article

Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

Insert

CALCOCO1 (CALCOCO1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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myc-POMP

Plasmid

#86764

Purpose

N-terminal myc-tagged protein expression in mammalian cells

Depositor

Do-Hyung Kim

Article

Yun et al Mol Cell. 2016 Feb 18;61(4):625-39. doi:

Insert

human POMP (POMP Human)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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C1-FKBP(F36V):mCherry-Akt2(K14A)

Plasmid

#72906

Purpose

Recruitable Akt2(K14A) mutant

Depositor

Ivan Yudushkin

Article

Ebner et al J Cell Biol. 2017 Jan 31. pii: jcb.20161

Insert

RAC-beta serine/threonine-protein kinase (AKT2 Human)

Use

Tags

FKBP(F36V), mCherry

Expression

Mammalian

Mutation

K14A mutation

Promoter

Available since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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EGFR∆550-580-mEGFP

Plasmid

#203788

Purpose

Number and brightness analysis of EGFR oligomerization

Depositor

Thorsten Wohland

Article

Balasubramanian et al Biophys J. 2022 Dec 6;121(23):4452-4466.

Insert

EGFR (EGFR Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

aa 550-580 deleted

Promoter

Available since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

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NoTag MAP1LC3C

Plasmid

#137762

Purpose

Expression of LC3C without epitope tags

Depositor

Carol Mercer

Article

Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

Insert

MAP1LC3C (MAP1LC3C Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag Jip1 JBD

Plasmid

#15751

Purpose

binds to Jnk

Depositor

Roger Davis

Article

Dickens et al Science. 1997 Aug 1. 277(5326):693-6.

Insert

Jip1 (Jnk binding domain) (Mapk8ip1 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

deleted 1-126 and 282-660

Promoter

Available since

April 1, 2014

Availability

Academic Institutions and Nonprofits only
pMM9-MAPKK2-KW71 [delta(48-55)/S222D/S226D]

Plasmid

#40806

Depositor

Natalie Ahn

Article

Whalen et al Mol Cell Biol. 1997 Apr;17(4):1947-58.

Insert

MAP2K2 (MAP2K2 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

delta(48-55) + S222D + S226D

Promoter

MSV-LTR

Available since

Nov. 14, 2012

Availability

Academic Institutions and Nonprofits only
pGEX-4T-1 3xHA-MKK6-EE

Plasmid

#47579

Purpose

Bacterial expression plasmid for GST-3xHA-tagged constitutively active MKK6

Depositor

Kevin Janes

Article

Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

Insert

MKK6-EE (MAP2K6 Human)

Use

Tags

3xHA and GST

Expression

Bacterial

Mutation

Ser207 to Glu, Thr211 to Glu

Promoter

Available since

Aug. 28, 2013

Availability

Academic Institutions and Nonprofits only
LentiCRISPR v2-sgAMPK alpha1 clone2

Plasmid

#162120

Purpose

Lentiviral sgRNA plasmid targeting human AMPK alpha1

Depositor

Boyi Gan

Article

Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

Insert

sgAMPK alpha1 (PRKAA1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pDSHA-ARL2-Myc

Plasmid

#67354

Purpose

Mammalian expression of hArl2 with C-term Myc tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL2 (ARL2 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
782 pMEActMEK

Plasmid

#31166

Depositor

Nathan Lawson

Article

Covassin et al Dev Biol. 2009 May 15. 329(2):212-26.

Insert

MEK (MAP2K1 Human)

Use

Entry vector

Tags

Expression

Mutation

Ser218Glu Ser222Asp; activated MEKK

Promoter

Available since

Aug. 11, 2011

Availability

Academic Institutions and Nonprofits only
pEGFP-Akt1(D323A/D325A)

Plasmid

#86630

Purpose

Expresses eGFP-tagged human Akt1(D323A/D325A) mutant

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt1(D323A/D325A) (AKT1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221-ARF6

Plasmid

#67314

Purpose

hArf6 with stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF6 (ARF6 Human)

Use

Gateway entry vector

Tags

Expression

Bacterial

Mutation

Promoter

None

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDSHA-ARL8A-Myc

Plasmid

#67361

Purpose

Mammalian expression of hArl8 with C-term Myc tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL8A (ARL8A Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
PKM-IRES-hrGFP

Plasmid

#73405

Purpose

Mammalian expression of mouse PKM and hrGFP

Depositor

Raymond Swanson

Article

Brennan-Minnella et al Cell Death Dis. 2013 Apr 4;4:e580. doi:

Insert

PKM (Prkcz Mouse)

Use

Tags

3xFLAG and pIRES-hrGFP-2a

Expression

Mammalian

Mutation

truncated, constitutively active form

Promoter

CMV

Available since

Feb. 19, 2016

Availability

Academic Institutions and Nonprofits only
pEBG-MKK4 (GST)

Plasmid

#21563

Depositor

Leonard Zon

Article

Sanchez et al Nature. . 372(6508):794-8.

Insert

mitogen-activated protein kinase kinase 4 (Map2k4 Mouse)

Use

Tags

GST

Expression

Mammalian

Mutation

Promoter

Available since

July 17, 2009

Availability

Academic Institutions and Nonprofits only
pFBD-Akt1(T308A/S473A)

Plasmid

#86578

Purpose

Expresses human full-length Akt1(T308A/S473A) phosphomutant

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt1 (AKT1 Human)

Use

Tags

10xHis

Expression

Insect

Mutation

T308A/S473A phosphomutant

Promoter

Available since

April 4, 2017

Availability

Academic Institutions and Nonprofits only

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pDSHA-ARF5-Myc

Plasmid

#67351

Purpose

Mammalian expression of hArf5 with C-term Myc tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF5 (ARF5 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pLenti6.3/V5-DEST-PKCi-A120E-ΔPB1

Plasmid

#56658

Purpose

expresses constitutively active human PKCi with a deletion on the PB1 domain

Depositor

Pedro Salas

Article

Forteza et al Biol Open. 2013 Oct 8;2(11):1264-9. doi:

Insert

Human PKCi (PRKCI Human)

Use

Lentiviral

Tags

V5/His

Expression

Mutation

A120E and deletion of PB1 domain (aa 24–98)

Promoter

CMV

Available since

Sept. 3, 2014

Availability

Academic Institutions and Nonprofits only