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pPRKCZ-C1-EGFP (C-PRKCZ-C1)

Plasmid

#217759

Purpose

Fluorescent reporter for ceramide (putative, mammalian expression)

Depositor

Paula Nunes-Hasler

Article

Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

Insert

Protein kinase C zeta (PRKCZ Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

C1 domain (aa 123-193)

Promoter

CMV

Available since

June 11, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pEGFP-PRKCZ-C1 (N-PRKCZ-C1)

Plasmid

#217760

Purpose

Fluorescent reporter for ceramide (putative, mammalian expression)

Depositor

Paula Nunes-Hasler

Article

Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

Insert

Protein kinase C zeta (PRKCZ Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

C1 domain (aa 123-193)

Promoter

CMV

Available since

June 11, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pPRKCZ-C20-EGFP (C-PRKCZ-C20)

Plasmid

#217761

Purpose

Fluorescent reporter for ceramide (putative, mammalian expression)

Depositor

Paula Nunes-Hasler

Article

Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

Insert

Protein kinase C zeta (PRKCZ Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

C20 domain (aa 405-592)

Promoter

CMV

Available since

June 11, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pEGFP-PRKCZ-C20 (N-PRKCZ-C20)

Plasmid

#217762

Purpose

Fluorescent reporter for ceramide (putative, mammalian expression)

Depositor

Paula Nunes-Hasler

Article

Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

Insert

Protein kinase C zeta (PRKCZ Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Linker GGSSGGGA plus C20 domain (aa 405-592)

Promoter

CMV

Available since

June 11, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pNES-PRKCZ-C20-EGFP (C-NES-PRKCZ-C20)

Plasmid

#217763

Purpose

Fluorescent reporter for ceramide (putative, mammalian expression)

Depositor

Paula Nunes-Hasler

Article

Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

Insert

Protein kinase C zeta (PRKCZ Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

NES (ALQKKLEELELDEAPVAT) plus C20 domain (aa 405-…

Promoter

CMV

Available since

June 11, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pRK-ATF3

Plasmid

#26115

Depositor

Yihong Ye

Article

Wang et al Proc Natl Acad Sci U S A. 2009 Feb 17. 1

Insert

ATF3 (ATF3 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 2, 2010

Availability

Academic Institutions and Nonprofits only

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pRK-ATF4

Plasmid

#26114

Depositor

Yihong Ye

Article

Wang et al Proc Natl Acad Sci U S A. 2009 Feb 17. 1

Insert

ATF4 (ATF4 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 2, 2010

Availability

Academic Institutions and Nonprofits only

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p1A Prk-

Plasmid

#162694

Purpose

p1A negative control expressing CbbLS and inactive Prk (Prk K20M S21A)

Depositor

David Savage

Article

Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

Insert

H. neapolitanus CbbLS and S. elongatus Prk

Use

Tags

N terminal 6x His tag on PRK

Expression

Bacterial

Mutation

Prk inactive (K20M S21A native protein, this map …

Promoter

PLtet0-1 promoter

Available since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pRK793

Plasmid

#8827

Depositor

David Waugh

Article

Kapust et al Protein Eng. 2001 Dec . 14(12):993-1000.

Insert

TEV protease, S219V mutant

Use

Tags

His, MBP (with TEV), and polyarginine

Expression

Bacterial

Mutation

S219V mutation (improves stability)

Promoter

Available since

May 24, 2006

Availability

Academic Institutions and Nonprofits only

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pLenti_PGK_Prkaa1

Plasmid

#204356

Purpose

Lentiviral plasmid, Expresses Prkaa1 under the control of the PGK promoter

Depositor

Günter Schneider

Insert

AMP-activated protein kinase alpha 1 (Prkaa1 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pRK603

Plasmid

#8831

Depositor

David Waugh

Article

Kapust et al Protein Expr Purif. 2000 Jul . 19(2):312

Insert

TEV protease, S219D mutant

Use

Tags

Expression

Bacterial

Mutation

S219D mutant

Promoter

Available since

June 22, 2006

Availability

Academic Institutions and Nonprofits only

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pRKO

Plasmid

#49456

Purpose

loxP-kanMX-loxP cassette inserted into the ACT1 intron for PCR amplification of the intron/kanMX module for one-step homology-driven genomic disruptions

Depositor

Marc Gartenberg

Article

Cheng et al Nucleic Acids Res. 2000 Dec 15;28(24):E1

Insert

loxP-kanMX-loxP cassette

Use

Cre/Lox

Tags

Expression

Bacterial

Mutation

Promoter

none

Available since

Dec. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pRK1043

Plasmid

#8835

Depositor

David Waugh

Article

Kapust et al Protein Eng. 2001 Dec . 14(12):993-1000.

Insert

tobacco etch virus protease, catalytic domain

Use

Tags

MBP and polyarginine

Expression

Bacterial

Mutation

Autolysis-resistant S219V mutant

Promoter

Available since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

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pLenti_C_Myc_DDK_IRES_Puro_PRKRA

Plasmid

#106113

Purpose

Lentiviral expression of PRKRA

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

PRKRA (PRKRA Human)

Use

Lentiviral

Tags

DDK (FLAG) and Myc

Expression

Mutation

Promoter

Available since

Feb. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_PRKACB_Pkinase

Plasmid

#110024

Purpose

Protein expression and purification of PRKACB_Pkinase

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

PRKACB_Pkinase (PRKACB Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pRK792

Plasmid

#8830

Depositor

David Waugh

Article

Kapust et al Protein Eng. 2001 Dec . 14(12):993-1000.

Insert

TEV protease, S219P mutant

Use

Tags

His, MBP (cleaved), and polyarginine

Expression

Bacterial

Mutation

S219P mutation

Promoter

Available since

July 18, 2006

Availability

Academic Institutions and Nonprofits only

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pRK1035

Plasmid

#8832

Depositor

David Waugh

Article

Nallamsetty et al Protein Expr Purif. 2004 Nov . 38(1):108

Insert

TVMV protease

Use

Tags

His and MBP (cleaved)

Expression

Bacterial

Mutation

synthetic gene with codons optimized for E. coli

Promoter

Available since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_DNAJB1_PRKACA

Plasmid

#205805

Purpose

Express mEGFP-tagged fusion protein, DNAJB1_PRKACA from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

DNAJB1_PRKACA (DNAJB1, PRKACA Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pRK7

Plasmid

#10883

Depositor

John Blenis

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 13, 2006

Availability

Academic Institutions and Nonprofits only

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pDONR223_PRKACA_WT

Plasmid

#82311

Purpose

Gateway Donor vector containing PRKACA , part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, Jesse Boehm, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PRKACA (PRKACA Human)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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