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Addgene

pGEX-4T-1 3xFlag-JNK1a1
(Plasmid #47574)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 47574 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pGEX-4T-1
  • Backbone manufacturer
    Amersham
  • Backbone size w/o insert (bp) 5053
  • Total vector size (bp) 6208
  • Modifications to backbone
    3xFlag tag inserted at BamHI site of pGEX-4T-1, regenerating the BamHI site downstream of 3xFlag, with a unique HindIII site added between the BamHI and EcoRI sites of pGEX-4T-1.
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    BL21
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    JNK1a1
  • Alt name
    MAPK8
  • Alt name
    SAPK1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1155
  • GenBank ID
    NM_002750
  • Entrez Gene
    MAPK8 (a.k.a. JNK, JNK-46, JNK1, JNK1A2, JNK21B1/2, PRKM8, SAPK1, SAPK1c)
  • Tags / Fusion Proteins
    • GST (N terminal on backbone)
    • 3xFlag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer pGEX 5'
  • 3′ sequencing primer pGEX 3'
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Addgene plasmid #13798.
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-4T-1 3xFlag-JNK1a1 was a gift from Kevin Janes (Addgene plasmid # 47574 ; http://n2t.net/addgene:47574 ; RRID:Addgene_47574)
  • For your References section:

    A high-throughput assay for phosphoprotein-specific phosphatase activity in cellular extracts. Bose AK, Janes KA. Mol Cell Proteomics. 2013 Mar;12(3):797-806. doi: 10.1074/mcp.O112.024059. Epub 2012 Dec 11. 10.1074/mcp.O112.024059 PubMed 23233447