C1-FKBP(F36V):mCherry-Akt2(K14A)
(Plasmid
#72906)
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PurposeRecruitable Akt2(K14A) mutant
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 72906 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneC1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRAC-beta serine/threonine-protein kinase
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Alt nameAkt2
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SpeciesH. sapiens (human)
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MutationK14A mutation
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Entrez GeneAKT2 (a.k.a. HIHGHH, PKBB, PKBBETA, PRKBB, RAC-BETA)
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Tag
/ Fusion Protein
- FKBP(F36V), mCherry (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (unknown if destroyed)
- 3′ cloning site BamHI (unknown if destroyed)
- 5′ sequencing primer TAACAACTCCGCCCCATT
- 3′ sequencing primer cctctacaaatgtggtatggc (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
C1-FKBP(F36V):mCherry-Akt2(K14A) was a gift from Ivan Yudushkin (Addgene plasmid # 72906 ; http://n2t.net/addgene:72906 ; RRID:Addgene_72906) -
For your References section:
Localization of mTORC2 activity inside cells. Ebner M, Sinkovics B, Szczygiel M, Ribeiro DW, Yudushkin I. J Cell Biol. 2017 Jan 31. pii: jcb.201610060. doi: 10.1083/jcb.201610060. 10.1083/jcb.201610060 PubMed 28143890