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pCI-neo VPS50-13Myc
Plasmid#175093Purpose13Myc tagged VPS50 is expressed in mammalian cellsDepositorInsertVPS50 (CCDC132 Human)
UseTags13MycExpressionMammalianMutationPromoterCMVAvailable sinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1-β4 hinge-ear
Plasmid#196692PurposeExpresses GST-AP-4 β4 hinge-ear (residues 534-739) in bacteriaDepositorInsertAP-4 β4 hinge-ear (534-739) (AP4B1 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEF6 VPS51-V5-6His
Plasmid#175502PurposeV5 tagged VPS51 is expressed in mammalian cellsDepositorInsertVPS51 (VPS51 Human)
UseTagsV5ExpressionMammalianMutationA661V- please see depositor commentsPromoterAvailable sinceDec. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOGG043
Plasmid#133123PurposeLevel 0 PU module synthesised as a fragment (pL0M-PU-PsNifH). Synthetic consensus promoter to drive nodule-specific expression in biovars and strains of R. leguminosarum.DepositorInsertPsNifH Synthetic consensus nifH promoter
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceDec. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR-GFP-WT1 +KTS C terminal HA
Plasmid#217640PurposeEntry vector with WT1 +KTS mutant with C terminal HA for gateway cloningDepositorInsertWT1 +KTS isoform (WT1 Human)
UseGateway cloningTagsHAExpressionMutationPromoterAvailable sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR-GFP-WT1 -KTS C terminal HA
Plasmid#217642PurposeEntry vector with WT1 -KTS mutant with C terminal HA for gateway cloningDepositorInsertWT1 -KTS isoform (WT1 Human)
UseGateway cloningTagsHAExpressionMutationp.408_410delKTSPromoterAvailable sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1 WT1 +1 ATG
Plasmid#217643PurposeDox inducible WT1 cDNA - as transcribed from +1ATG (long isoform)DepositorInsertWT1 long isoform (WT1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1 WT1 +378 ATG
Plasmid#217644PurposeDox inducible WT1 cDNA - as transcribed from +378ATG (short isoform)DepositorInsertWT1 short isoform (WT1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1 Ex8 mut WT1
Plasmid#217645PurposeDox inducible WT1 cDNA with exon 8 mutationDepositorInsertWT1 (WT1 Human)
UseTagsExpressionMammalianMutationFrameshift exon 8PromoterAvailable sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1 Ex9 mut WT1
Plasmid#217646PurposeDox inducible WT1 cDNA with exon 9 mutationDepositorInsertWT1 (WT1 Human)
UseTagsExpressionMammalianMutationFrameshift exon 9PromoterAvailable sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGBT9-GGA2-GAE
Plasmid#199175PurposeExpression of GAL4 DNA-binding domain (BD)-GGA2-GAE domain fusion protein in yeast (yeast two-hybrid assays)DepositorInsertGGA2-GAE domain (GGA2 Human)
UseTagsGAL4-DNA binding domain fragmentExpressionYeastMutationContains Lys 545 Gln substitutionPromoterADH1Available sinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGBT9-GGA3-GAE
Plasmid#199176PurposeExpression of GAL4 DNA-binding domain (BD)-GGA3-GAE domain fusion protein in yeast (yeast two-hybrid assays)DepositorInsertGGA3-GAE domain (GGA3 Human)
UseTagsGAL4-DNA binding domain fragmentExpressionYeastMutationPromoterADH1Available sinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGBT9-GGA1-GAE
Plasmid#199174PurposeExpression of GAL4 DNA-binding domain (BD)-GGA1-GAE domain fusion protein in yeast (yeast two-hybrid assays)DepositorInsertGGA1-GAE domain (GGA1 Human)
UseTagsGAL4-DNA binding domain fragmentExpressionYeastMutationPromoterADH1Available sinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGST-parallell2-GGA3(571-723)
Plasmid#199150PurposeExpresses GST-GGA3(571-723) in bacteriaDepositorInsertGGA3(571-723) (GGA3 Human)
UseTagsGST and TEV protease cleavage siteExpressionBacterialMutationPromotertacAvailable sinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGAD424-Rabex-5
Plasmid#197043PurposeExpression of GAL4 transcriptional activation domain (AD)-Rabex-5 fusion protein in yeast (yeast two-hybrid assays)DepositorInsertRabex-5 (RABGEF1 Bovine)
UseTagsGAL4 transcriptional activation domain (AD) fragm…ExpressionYeastMutationPromoterADH1Available sinceMarch 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pACT2-μ1B (137-423)
Plasmid#198172PurposeExpression of GAL4 transcriptional activation domain (AD)-AP-1 μ1B (137-423) fusion protein in yeast (yeast two-hybrid assays)DepositorInsertAP-1 μ1B (137-423)
UseTagsGAL4 transcriptional activation domain (AD) fragm…ExpressionYeastMutationPromoterADH1Available sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGBT9-AAGAB
Plasmid#198332PurposeExpression of GAL4 DNA-binding domain (BD)-AAGAB fusion protein in yeast (yeast two-hybrid assays)DepositorInsertAAGAB (AAGAB Human)
UseTagsGAL4-DNA binding domain (BD) fragmentExpressionYeastMutationPromoterADH1Available sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP-HA-BACE1
Plasmid#165032PurposeHA-BACE1DepositorInsertBACE1 (BACE1 Human)
UseTagsHA (embedded after propeptide cleavage site)ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lamp1-mCherry-RUSH
Plasmid#202799PurposeRUSH cargo: Str-KDEL_IRES_SBP-mCherry-Lamp1DepositorInsertLamp1 (Lamp1 Rat)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceAug. 18, 2023AvailabilityAcademic Institutions and Nonprofits only
