pGST-parallell2-GGA3(571-723)
(Plasmid
#199150)
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PurposeExpresses GST-GGA3(571-723) in bacteria
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 199150 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepGST-paralle2
- Backbone size w/o insert (bp) 5037
- Total vector size (bp) 5499
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameGGA3(571-723)
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SpeciesH. sapiens (human)
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Insert Size (bp)462
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Entrez GeneGGA3
- Promoter tac
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Tags
/ Fusion Proteins
- GST (N terminal on backbone)
- TEV protease cleavage site (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamH1 (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer GST forward
- 3′ sequencing primer GST reverse (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGST-parallell2-GGA3(571-723) was a gift from Juan Bonifacino (Addgene plasmid # 199150 ; http://n2t.net/addgene:199150 ; RRID:Addgene_199150) -
For your References section:
Recognition of accessory protein motifs by the gamma-adaptin ear domain of GGA3. Miller GJ, Mattera R, Bonifacino JS, Hurley JH. Nat Struct Biol. 2003 Aug;10(8):599-606. doi: 10.1038/nsb953. 10.1038/nsb953 PubMed 12858162
Map uploaded by the depositor.
