pGEX-4T-1-β4 hinge-ear
(Plasmid #196692)

• Purpose: Expresses GST-AP-4 β4 hinge-ear (residues 534-739) in bacteria
• Depositing Lab: Juan Bonifacino
• Publication: Mattera et al J Biol Chem. 2015 Dec 25;290(52):30736-49. doi: 10.1074/jbc.M115.683409. Epub 2015 Nov 5.

Backbone

• Vector backbone: pGEX-4T-1
• Backbone size w/o insert (bp): 4969
• Total vector size (bp): 5604
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: AP-4 β4 hinge-ear (534-739)
• Species: H. sapiens (human)
• Insert Size (bp): 639
• Entrez Gene: AP4B1 (a.k.a. BETA-4, CPSQ5, SPG47)
• Promoter: tac
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SmaI (destroyed during cloning)
• 3′ cloning site: SmaI (destroyed during cloning)
• 5′ sequencing primer: GST forward
• 3′ sequencing primer: GST reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Contains 15 extra nucleotides encoding sequence MAMEA at 5' of insert