Bivalent Motif-Ear Interactions Mediate the Association of the Accessory Protein Tepsin with the AP-4 Adaptor Complex.
Mattera R, Guardia CM, Sidhu SS, Bonifacino JS
J Biol Chem. 2015 Dec 25;290(52):30736-49. doi: 10.1074/jbc.M115.683409. Epub 2015 Nov 5. PubMed Article
Mattera R, Guardia CM, Sidhu SS, Bonifacino JS
J Biol Chem. 2015 Dec 25;290(52):30736-49. doi: 10.1074/jbc.M115.683409. Epub 2015 Nov 5. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 196583 | pcDNA 3.1-TSF-ε | Expresses the two Strep/one FLAG (TSF)-tagged human AP-4 ε subunit in mammalian cells |
| 196636 | pcDNA 3.1-TSF-tepsin | Expresses the two Strep/one FLAG (TSF)-tagged human tepsin in mammalian cells |
| 196647 | pGBT9-tepsin | Expression of GAL4 DNA-binding domain (BD)-tepsin fusion protein |
| 196670 | pGEX-4T-1-ε ear | Expresses GST-AP-4 ε ear (residues 840-1137) in bacteria |
| 196692 | pGEX-4T-1-β4 hinge-ear | Expresses GST-AP-4 β4 hinge-ear (residues 534-739) in bacteria |
| 196707 | pEGFP-C2-tepsin | Expresses GFP-tepsin in mammalian cells |
| 196728 | pEGFP-N1-tepsin | Expresses tepsin-GFP in mammalian cells |