pEGFP-C2-tepsin
(Plasmid #196707)

• Purpose: Expresses GFP-tepsin in mammalian cells
• Depositing Lab: Juan Bonifacino
• Publication: Mattera et al J Biol Chem. 2015 Dec 25;290(52):30736-49. doi: 10.1074/jbc.M115.683409. Epub 2015 Nov 5.

Backbone

• Vector backbone: pEGFP-C2
• Backbone size w/o insert (bp): 4736
• Total vector size (bp): 6309
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: tepsin
• Species: H. sapiens (human)
• Insert Size (bp): 1578
• Mutation: silent substitution in codon 199 to eliminate internal EcoRI site
• Entrez Gene: TEPSIN (a.k.a. C17orf56, ENTHD2)
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: SalI (destroyed during cloning)
• 5′ sequencing primer: GFP-C forward
• 3′ sequencing primer: GFP-C reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry