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Plasmid#205614PurposeExpression of human TRKB receptor tyrosine kinase in mammalian cellsDepositorInsertTRKB receptor tyrosine kinase (NTRK2 Human)
UseTagsV5-HisExpressionMammalianMutationPromoterCMVAvailable sinceSept. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-3xHA-Puro
Plasmid#207729PurposeEmpty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a 3xHA-2A-Puro cassetteDepositorTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFastBac His6 TEV LIC cloning vector (4B)
Plasmid#30115DepositorTypeEmpty backboneUseTagsHis6-TEVExpressionInsectMutationPromoterAvailable sinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGL403
Plasmid#119953PurposeExpresses GPPS and LimS, for the production of GPP and (S)-limonene, in Escherichia coli.DepositorInsertsgpps
limS
UseTagsExpressionBacterialMutationN-terminal truncation of 56 amino acid residues (…PromoterAvailable sinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
Inducible tdTomato
Plasmid#183718PurposetdTomato expression from dox-inducible promoter; plasmid encodes the TetR inducerDepositorInsertstdTomato
rTetR
UseLentiviralTagsExpressionMutationPromoterTRE3GV and UbiCAvailable sinceMay 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pL-U6-sgRNA-SFFV-Puro-P2A-EGFP
Plasmid#175037PurposeLentiviral delivery of sgRNA. Expresses PuroR and eGFP from SFFV promoter.DepositorInsertSp sgRNA scaffold
UseLentiviralTagsExpressionMutationPromoterU6Available sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
BRAF in pENTR
Plasmid#216605Purposegateway cloning donor vector containing BRAFDepositorInsertBRAF (BRAF Human)
UseGateway entry plasmidTagsExpressionMutationPromoterAvailable sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-sfGFP-3xP3-RFP
Plasmid#127566PurposeCRISPaint universal donor plasmid for gene exon insertion. Encodes sfGFP-SV40 and 3xP3-RFP visible eye marker.DepositorInsertsfGFP
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceJuly 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAC-LYCipi
Plasmid#53279PurposeContains crtE, crtB, crtI, and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and thereby produces lycopene in Escherichia coliDepositorInsertidi
UseLow copy number bacterial cloning vectorTagsExpressionMutationPromoterendogenous promotersAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGal4AD-CIB1
Plasmid#28245PurposeExpresses Gal4 Activation Domain fused to CIB1 for blue-light-induced transcription activationDepositorUseTagsCIB1ExpressionYeastMutationPromoterADH1Available sinceMarch 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCas9_sgRNA_0
Plasmid#70763Purposeexpresses Ustilago maydis codon-optimized Cas9, contains U. maydis U6 promoter, is self-replicatingDepositorInsertsU6 promoter
cas9
tnos terminator
UseSelf-replicating in ustilago maydis, conferring c…TagsN-terminal NLS, C-terminal HA-tag +NLSExpressionMutationStreptococcus pyogenes gene codon-optimized for U…PromoterU6 from Ustilago maydis and synthetic otef promot…Available sinceNov. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
USP21 (3I3T)
Plasmid#25147PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertusp21.209.563 (USP21 Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceAug. 6, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET21a-ValRS-His
Plasmid#124122PurposeBacterial expression plasmid for PURE system componentsDepositorInsertValyl-tRNA ligase (valS Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
GFP-Intersectin Short
Plasmid#47394DepositorInsertIntersectin short (ITSN1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO eNpHR 3.0-EYFP (AAV Retrograde)
Viral Prep#26966-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV-Ef1a-DIO eNpHR 3.0-EYFP (#26966). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO eNpHR 3.0-EYFP plasmid DNA. EF1a-driven, cre-dependent eNpHR 3.0-EYFP for optogenetic inhibition. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsEYFP (Cre-dependent)Available sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET29b-clbO
Plasmid#114153PurposeExpresses ClbODepositorInsertclbO
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET11a-Z-NGFP
Plasmid#52734PurposeIPTG-inducible expression of Z-NGFP positive control for in vivo split GFP assembly assayDepositorInsertNGFP+Z-fusion
UseTagsHis tagExpressionBacterialMutationPromoterT7Available sinceJune 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV hSyn FLEx-FRT hM3Dq-mCherry
Plasmid#161575PurposeExpression of hM3Dq-mCherry in a Flp-dependent fashionDepositorInserthM3Dq-mCherry
UseAAVTagsExpressionMutationNAPromoterAvailable sinceNov. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 HRPT2
Plasmid#11048DepositorInsertHRPT2 (CDC73 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
4QQD
Plasmid#61947PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertUHRF1:SDC130-H02:C39674 (UHRF1 Human)
UseTags6xHis and MHHHHHHSSGRENLYFQGExpressionBacterialMutationSee commentsPromoterT7Available sinceMarch 2, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits