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Plasmid#110161PurposeExpression of human eEF2K (S441A/S445A) in mammalian cellsDepositorInserteEF2K (eukaryotic elongation factor 2 kinase) (EEF2K Human)
UseTagsHAExpressionMammalianMutationS441A/S445APromoterCMVAvailable sinceMay 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHR-flag-GFP-NCAM-1
Plasmid#205190PurposeThis vector encodes of a synCAM (NCAM1) and GFPDepositorInsertsynCAM NCAM1 and GFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-Camk2-NES-Twitch-GR
Plasmid#173044PurposeTnC-based calcium sensor with green-red FRET pair ratiometric readoutDepositorInsertNES-Twitch-GR
UseTagsExpressionMammalianMutationPromoterCamK2Available sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
mOrange pHis-//
Plasmid#53304PurposeEmpty vector to express N-terminal His-mOrange-tagged fusion protein.DepositorTypeEmpty backboneUseTagsHis and mOrangeExpressionBacterialMutationPromoterAvailable sinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW ymScarletI
Plasmid#118452PurposeOverexpression of yeast codon-optimized mScarletIDepositorInsertymScarletI
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVITRO-HPV56 L1L2
Plasmid#52585PurposeExpresses HPV56 L1 and L2 in mammalian cellsDepositorInsertsHPV56 L1
HPV56 L2
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn Con/Fon hChR2(H134R)-EYFP (AAV Retrograde)
Viral Prep#55645-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV-hSyn Con/Fon hChR2(H134R)-EYFP (#55645). In addition to the viral particles, you will also receive purified pAAV-hSyn Con/Fon hChR2(H134R)-EYFP plasmid DNA. Synapsin-driven, Cre-dependent and Flp-dependent Channelrhodopsin-EYFP expression for optogenetic activation. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsEYFP (Cre- and Flp-dependent)Available sinceDec. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
Ai148 (TIT2L-GC6f-ICL-tTA2) targeting vector
Plasmid#114428PurposeTarget a Cre-dependent GCaMP6f expression and a tTA2 cassette into the mouse TIGRE locusDepositorInsertGCaMP6f
UseCre/Lox and Mouse TargetingTagsExpressionMutationPromoterTRE2, CAGAvailable sinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pU6-sp-sgRNA-RNF2_+41nick
Plasmid#135958PurposeS. pyogenes sgRNA for +41 nick in RNF2DepositorInsertRNF2_+41 nicking sgRNA
UseTagsExpressionMammalianMutationSee manuscriptPromoterU6Available sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMJ922
Plasmid#78312PurposeExpression of His6-MBP-tagged Cas9-NLS-EGFP protein in E. coliDepositorInsertSpCas9
UseTagsHA-2xNLS-EGFP-NLS and His6-MBP-TEVExpressionBacterialMutationhuman codon-optimized gene sequencePromoterT7Available sinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
AAV-GfaABC1D-lck-smMyc-4x6T-WPRE
Plasmid#196415PurposeAstrocytic expression of membrane-targeted Myc spaghetti monster reporter in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassetteDepositorInsertLck-smMyc
UseAAV; Astrocyte-selectiveTagsLckExpressionMammalianMutationPromoterCAG and GfaABC1DAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SCP1-dSa VPR mini.-2X snRP-1 Hbb
Plasmid#99692PurposeExpresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Hbb, vector allows for strong activation of mouse Hbb, can be packaged and delivered as AAVDepositorArticleInsertdCas9 and gRNA targeting Hbb (Hbb Synthetic)
UseAAVTagsVPR miniExpressionMutationdead Cas9PromoterAvailable sinceJan. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
1313_pAAV-U6-SA-BbsI-MluI-gRNA-HLP-SACas9-HA-OLLAS-spA
Plasmid#109314PurposePlasmid for liver-specific expression of AAV SaCas9 with a BbsI cloning site for a gRNADepositorTypeEmpty backboneUseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
Flag-HA-USP21
Plasmid#22574DepositorInsertUSP21 (USP21 Human)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationNonePromoterAvailable sinceNov. 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-SIGLEC7-Fc(DAPA)-AviTag-6xHis
Plasmid#156773PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertSIGLEC7 (SIGLEC7 Human)
UseTagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX459V2.0-eSpCas9(1.1)
Plasmid#108292PurposepX459 V2.0 (Plasmid #62988) with the K848A, K1003A, and R1060A mutationsDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMay 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
FRE5
Plasmid#62377PurposeFluorescent reporter to quantify the ratio of two alternative splicing patterns.DepositorInsertdsRed and GFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET32a-HD46Q
Plasmid#11515DepositorInserthuntingtin, exon 1, 46Q glutamines (HTT Human)
UseTagsHis, His (out of frame), and TrxExpressionBacterialMutationhuntingtin exon 1 with 46 glutamines, vector m…PromoterAvailable sinceApril 28, 2006AvailabilityAcademic Institutions and Nonprofits only -
p45sheLL
Plasmid#37323DepositorInsertHPV45L1+L2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
HiBiT-FZD6
Plasmid#195844PurposeExpresses human FZD6 with an N-terminal HiBiT; contains M345LDepositorInsertFZD6 (FZD6 Human)
UseTagsHiBiTExpressionMammalianMutationM345LPromoterCMVAvailable sinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only