Addgene: Gene Knock-Ins in Drosophila Using Homology-Independent Insertion of Universal Donor Plasmids.

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Norbert Perrimon
Bosch et al

Gene Knock-Ins in Drosophila Using Homology-Independent Insertion of Universal Donor Plasmids.
Bosch JA, Colbeth R, Zirin J, Perrimon N
Genetics. 2019 Nov 4. pii: genetics.119.302819. doi: 10.1534/genetics.119.302819. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
127553	pCFD3-frame_selector_0	Drosophila expression of frame selector sgRNA 0 that targets CRISPaint site for homology-independent knock-in
127554	pCFD3-frame_selector_1	Drosophila expression of frame selector sgRNA 1 that targets CRISPaint site for homology-independent knock-in
127555	pCFD3-frame_selector_2	Drosophila expression of frame selector sgRNA 2 that targets CRISPaint site for homology-independent knock-in
127556	pCRISPaint-T2A-Gal4-3xP3-RFP	CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Gal4-SV40 and 3xP3-RFP visible eye marker.
127557	pCRISPaint-T2A-sfGFP-3xP3-RFP	CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-sfGFP-SV40 and 3xP3-RFP visible eye marker.
127558	pCRISPaint-T2A-LexGAD-3xP3-RFP	CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-LexGAD-SV40 and 3xP3-RFP visible eye marker.
127559	pCRISPaint-T2A-QF2-3xP3-RFP	CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-QF2-SV40 and 3xP3-RFP visible eye marker.
127560	pCRISPaint-T2A-Cas9-T2A-GFP-3xP3-RFP	CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Cas9-T2A-GFP-SV40 and 3xP3-RFP visible eye marker.
127561	pCRISPaint-T2A-FLPo-3xP3-RFP	CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-FLPo-SV40 and 3xP3-RFP visible eye marker.
127562	pCRISPaint-T2A-Gal80-3xP3-RFP	CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Gal80-SV40 and 3xP3-RFP visible eye marker.
127563	pCRISPaint-T2A-Nluc-3xP3-RFP	CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Nluc-SV40 and 3xP3-RFP visible eye marker.
127564	pCRISPaint-T2A-Gal4DBD-3xP3-RFP	CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Gal4DBD-SV40 and 3xP3-RFP visible eye marker.
127565	pCRISPaint-T2A-p65-3xP3-RFP	CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-p65-SV40 and 3xP3-RFP visible eye marker.
127566	pCRISPaint-sfGFP-3xP3-RFP	CRISPaint universal donor plasmid for gene exon insertion. Encodes sfGFP-SV40 and 3xP3-RFP visible eye marker.
130276	pCRISPaint-3xP3-RFP	CRISPaint universal donor plasmid for gene exon insertion. Encodes 3xP3-RFP visible eye marker and multiple cloning site.
130277	pCRISPaint-3xP3-GFP	CRISPaint universal donor plasmid for gene exon insertion. Encodes 3xP3-GFP visible eye marker and multiple cloning site.
130278	pCFD3-Act5c	Drosophila expression of sgRNA that targets Actin5c CDS close to stop codon. Positive control sgRNA for CRISPaint knock-ins in cultured cells.
131152	pCFD5-frame_selector_0,1,2	Drosophila expression of frame selector sgRNAs 0,1, and 2 that target the CRISPaint site for homology-independent knock-in

Antibodies from Article