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pET-24a-ELP[V-150]

Plasmid

#67015

Purpose

Expresses ELP[V-150] in E. coli

Depositor

Ashutosh Chilkoti

Article

Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

Insert

ELP[V-150]

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pMRBAD-Z-CGFP

Plasmid

#52735

Purpose

L-(+)-arabinose-inducible expression of Z-CGFP positive control for in vivo split GFP assembly assay

Depositor

Lynne Regan

Article

Magliery et al J Am Chem Soc. 2005 Jan 12;127(1):146-57

Insert

Z+CGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

PBAD

Available since

May 7, 2014

Availability

Academic Institutions and Nonprofits only

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pCI-SEP-NRI

Plasmid

#23999

Depositor

Robert Malinow

Article

Kopec et al J Neurosci. 2006 Feb 15. 26(7):2000-9.

Insert

NR1 (Grin1 Rat)

Use

Tags

NR1 signal sequence (aa1-20) and SEP (supereclipt…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 5, 2010

Availability

Academic Institutions and Nonprofits only

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pET19b+H2B

Plasmid

#157809

Purpose

Expression of human histone H2B

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

H2B

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pST1374-NLS-flag-linker-Cas9

Plasmid

#44758

Depositor

Xingxu Huang

Article

Shen et al Cell Res. 2013 Apr 2. doi: 10.1038/cr.20

Insert

Human codon optimized Cas9

Use

CRISPR

Tags

32 amino acid linker, Flag, and NLS

Expression

Mammalian

Mutation

codon-optimized

Promoter

CMV

Available since

April 24, 2013

Availability

Academic Institutions and Nonprofits only

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GFP-TCF4

Plasmid

#109144

Purpose

For the expression of GFP-TCF in mammalian cells

Depositor

Mariann Bienz

Article

Townsley et al Nat Cell Biol. 2004 Jul;6(7):626-33. doi

Insert

TCF7L2 (TCF7L2 Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW ytdTomato

Plasmid

#118456

Purpose

Overexpression of yeast codon-optimized tdTomato

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ytdTomato

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pMiniT:SEP-GluA2 TKIT donor

Plasmid

#169421

Purpose

Donor DNA for TKIT knockin of SEP to GluA2 in mouse

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

Super ecliptic pHluorin (SEP)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pYLsgRNA-AtU3d

Plasmid

#66200

Purpose

cloning of sgRNAs for expression in plants

Depositor

Yao-Guang Liu

Article

Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

Type

Empty backbone

Use

Cloning vector

Tags

Expression

Mutation

Promoter

AtU3d

Available since

Aug. 10, 2015

Availability

Academic Institutions and Nonprofits only

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SQ171/pCSacB/ptRNA100

Bacterial Strain

#155206

Purpose

SQ171 strain supported by pCSacB and ptRNA100 plasmids

Depositor

Alexander Mankin

Article

Huang et al Nat Commun. 2020 Jun 9;11(1):2900. doi:

Bacterial Resistance

Kanamycin

Available since

April 27, 2021

Availability

Academic Institutions and Nonprofits only
pdCas9-sgRNA-RFP

Plasmid

#166005

Purpose

Bacterial expression of dCas9 (aTc control) and sgRNA (arabinose control)

Depositor

Martin Buck

Article

Bradley et al Biotechnol Rep (Amst). 2021 Oct 9;32:e00

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 27, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn FLEx-FRT hM4Di-mCherry

Plasmid

#161576

Purpose

Expression of hM4Di-mCherry in a Flp-dependent fashion

Depositor

Kevin Beier, Robert Malenka

Article

Beier et al Nature. 2017 Sep 21;549(7672):345-350. d

Insert

hM4Di-mCherry

Use

AAV

Tags

Expression

Mutation

NA

Promoter

Available since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

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SnFR-gamma8

Plasmid

#165496

Purpose

Green fluorescent glutamate reporter fused to TARP gamma-8 for mammalian expression.

Depositor

Andrew Plested

Article

Hao et al eLife. 2023 Jan 9;12:e84029. doi: 10.755

Insert

iGluSnFR extracellular domain fused to gamma-8 via NETO2 TM domain (Cacng8 Rat, Synthetic)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 30, 2021

Availability

Academic Institutions and Nonprofits only

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BirA in pET28a (w400-2)

Plasmid

#26624

Depositor

Eric Campeau

Insert

BirA

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

April 27, 2011

Availability

Academic Institutions and Nonprofits only

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UbcH5B

Plasmid

#60443

Purpose

Express E2 UbcH5B in E. Coli

Depositor

Cynthia Wolberger

Article

Wiener et al Nat Struct Mol Biol. 2013 Sep;20(9):1033

Insert

UbcH5B (UBE2D2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 29, 2015

Availability

Academic Institutions and Nonprofits only
GFP-(SIM)3

Plasmid

#126955

Purpose

Bacterial expression plasmid for eGFP-(SIM)3 client to assess SUMO-SIM scaffolds.

Depositor

Michael Rosen

Article

Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

Insert

EGFP-SUMO interacting motif (SIM) from PIASx (PIAS2 Human)

Use

Tags

eGFP

Expression

Bacterial

Mutation

Promoter

unknown

Available since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pCAGGs-BFP

Plasmid

#127348

Purpose

Expresses BFP constitutively via the CAGGS promoter in mammalian cells

Depositor

Phil Sharp

Insert

Blue Fluorescent Protein

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAGGS Promoter

Available since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pICSL01009::AtU6p

Plasmid

#46968

Purpose

Encodes the Arabidopsis U6 promoter in a level 0 vector (Weber et al. PLoS One 6:e16765, 2011). It is used to place an sgRNA uder the Arabidopsis U6 promoter.

Depositor

Sophien Kamoun

Article

Nekrasov et al Nat Biotechnol. 2013 Aug 8;31(8):691-3.

Insert

AtU6p

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 15, 2013

Availability

Academic Institutions and Nonprofits only

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TFORF0094

Plasmid

#142458

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

ZEB2 (ZEB2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

June 20, 2023

Availability

Academic Institutions and Nonprofits only

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pTol2p2A--3mnx1:mTagBFP-NS-MCS

Plasmid

#78250

Purpose

Can be used to subclone in gene of interest into blue motor neuron expression vector.

Depositor

Nicholas Cole

Article

Don et al Zebrafish. 2016 Sep 15.

Inserts

-3mnx1 (mnx1 Zebrafish)
-mTagBFP-NS-MCS

Use

Zebrafish motor neuron expression

Tags

Expression

Mutation

Promoter

-3mnx1

Available since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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