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Showing: 10901 - 10920 of 154801 results

  5. DD-Cas9 with filler sequence and Venus (EDCPV)

    Plasmid
    #90085
    Purpose
    This plasmid contains destabilized Cas9 and has Venus after P2A sequence. This vector also contains filler sequence which required to be remove for cloning of desired sgRNA
    Depositor
    Raffaella Sordella
    Article
    Senturk et al Nat Commun. 2017 Feb 22;8:14370. doi: 10
    Insert
    Cas9
    Use
    CRISPR and Lentiviral
    Tags
    Destabilized Domain and Flag
    Expression
    Mammalian
    Mutation
    Promoter
    EFS
    Available since
    July 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    90085-plasmid-map-sequence-id-182691

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  10. pAAV-Ef1a-Con/Foff 2.0-mCherry (AAV8)

    Viral Prep
    #137133-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-Ef1a-Con/Foff 2.0-mCherry (#137133). In addition to the viral particles, you will also receive purified pAAV-Ef1a-Con/Foff 2.0-mCherry plasmid DNA. EF1a-driven, Cre-dependent expression of mCherry (inhibited in presence of Flp). These AAV preparations are suitable purity for injection into animals.
    Depositor
    INTRSECT 2.0 Project, Karl Deisseroth
    Article
    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)
    Promoter
    EF1a
    Tags
    mCherry (Cre-dependent)
    Available since
    April 5, 2021
    Availability
    Academic Institutions and Nonprofits only

  16. JI401: Ad5 E1A/Hexon qRT-PCR standard

    Plasmid
    #131753
    Purpose
    Plasmid standard containing regions of the Ad5 E1A and Ad5 Hexon genes to create a qRT-PCR standard curve for detection of replication competent adenovirus (RCA).
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Inserts
    Ad5 E1A gene fragment
    Ad5 Hexon gene fragment
    Use
    Standard for qrt-pcr
    Tags
    Expression
    Mutation
    Promoter
    N/A
    Available since
    Oct. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    131753-plasmid-map-sequence-id-314394

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  17. pFastBac1-His10TEV

    Plasmid
    #159427
    Purpose
    empty pFastBac1 vector (polyhedrin promoter) adds TEV-protease-removable N-terminal His10-tag; avoids unwanted residues in the final protein sequence by using the BseRI restriction sites
    Depositor
    Debra Hansen
    Article
    Olson et al Protein Expr Purif. 2021 May 7:105890. d
    Type
    Empty backbone
    Use
    Tags
    TEV-protease-cleavable His10-tag
    Expression
    Insect
    Mutation
    Promoter
    polyhedrin
    Available since
    June 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    159427-plasmid-map-sequence-id-333995

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  18. pICherryNeo

    Plasmid
    #52119
    Purpose
    a bicistronic IRES-mCherry containing vector for mammalian expression (CMV promoter)
    Depositor
    Dario Vignali
    Type
    Empty backbone
    Use
    Tags
    IRES-mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    52119-plasmid-map-sequence-id-80505

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Showing: 10901 - 10920 of 154801 results