Addgene

We narrowed to 2,255 results for: PRK

Showing: 981 - 1000 of 2255 results

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pFIN-RK-GFP-WPRE

Plasmid

#44358

Depositor

Susan Semple-Rowland

Article

Semple-Rowland et al Mol Vis. 2010 May 27;16:916-34.

Insert

RK-GFP (MAPK14 Synthetic, Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

human rhodopsin kinase (pos 2557-2853)

Available since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR v2-sgAMPK alpha2 clone1

Plasmid

#162122

Purpose

Lentiviral sgRNA plasmid targeting human AMPK alpha2

Depositor

Boyi Gan

Article

Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

Insert

sgAMPK alpha2 (PRKAA2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX4T3-HA-ERK2 D321N

Plasmid

#53202

Purpose

Expresses GST and HA tagged human ERK2 D321N from bacterial expression vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

ERK2 (MAPK1 Human)

Use

Tags

GST and HA

Expression

Bacterial

Mutation

D321N

Promoter

Available since

June 24, 2014

Availability

Academic Institutions and Nonprofits only
pGEX4T3-HA-ERK2 GOF

Plasmid

#53200

Purpose

Expresses GST and HA tagged human ERK2 R67S D321N from bacterial expression vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

ERK2 (MAPK1 Human)

Use

Tags

GST and HA

Expression

Bacterial

Mutation

R67S/D321N

Promoter

Available since

June 24, 2014

Availability

Academic Institutions and Nonprofits only
FUW TetO HA-AMPKa1 AS

Plasmid

#69825

Purpose

Expresses AMPK alpha 1 in mammalian cells in a doxycycline inducible manner

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

5'-AMP-activated protein kinase catalytic subunit alpha-1 (PRKAA1 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

minimal CMV promoter with tetracycline operator

Available since

May 12, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV5 Flag p38 alpha

Plasmid

#20786

Depositor

Roger Davis

Article

Raingeaud et al J Biol Chem. 1995 Mar 31. 270(13):7420-6

Insert

pCMV5 Flag p38 alpha (Mapk14 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

April 14, 2009

Availability

Academic Institutions and Nonprofits only
PKN1 gRNA (BRDN0001162227)

Plasmid

#76276

Purpose

3rd generation lentiviral gRNA plasmid targeting human PKN1

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PKN1 (PKN1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PKN2 gRNA (BRDN0001147750)

Plasmid

#77926

Purpose

3rd generation lentiviral gRNA plasmid targeting human PKN2

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PKN2 (PKN2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

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pSLIK 3XFLAG-MKK7-EE-NLS hygro

Plasmid

#87780

Purpose

Inducible expression of constitutively active mutant MKK7 with a C-terminal NLS sequence

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

MKK7 (Map2k7 Mouse)

Use

Lentiviral

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

Promoter

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only
TFORF1165

Plasmid

#142743

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

PRKCD (PRKCD Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

June 8, 2023

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR v2-sgAMPK alpha2 clone2

Plasmid

#162123

Purpose

Lentiviral sgRNA plasmid targeting human AMPK alpha2

Depositor

Boyi Gan

Article

Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

Insert

sgAMPK alpha2 (PRKAA2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR v2-sgAMPK alpha2 clone3

Plasmid

#162124

Purpose

Lentiviral sgRNA plasmid targeting human AMPK alpha2

Depositor

Boyi Gan

Article

Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

Insert

sgAMPK alpha2 (PRKAA2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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PKN2 gRNA (BRDN0001149537)

Plasmid

#77927

Purpose

3rd generation lentiviral gRNA plasmid targeting human PKN2

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PKN2 (PKN2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PKN2 gRNA (BRDN0001487065)

Plasmid

#77928

Purpose

3rd generation lentiviral gRNA plasmid targeting human PKN2

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PKN2 (PKN2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PKN1 gRNA (BRDN0001149250)

Plasmid

#76275

Purpose

3rd generation lentiviral gRNA plasmid targeting human PKN1

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PKN1 (PKN1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PKN1 gRNA (BRDN0001148556)

Plasmid

#76277

Purpose

3rd generation lentiviral gRNA plasmid targeting human PKN1

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PKN1 (PKN1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG-eSpCas9-2A-GFP-sgRNA-DNA-PKcs

Plasmid

#220493

Purpose

To generate DNA-PKcs KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against DNA-PKcs exon 1.

Depositor

Sébastien Britton

Article

Bossaert et al bioRxiv [Preprint]. 2024 Jan 4:2024.01.0

Insert

sgRNA targeting DNA-PKcs (PRKDC) exon 1 (PRKDC Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

July 3, 2024

Availability

Academic Institutions and Nonprofits only

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pLentiPGK Puro DEST p38KTRClover

Plasmid

#59152

Purpose

Lentiviral vector to express p38 KTR mClover under PGK promoter (With Puromycin Resistance)

Depositor

Markus Covert

Article

Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

Insert

p38 Kinase Translocation Reporter (MAPK14 Mouse, Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Sept. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pBABEpuro-HA-ERK2 GOF

Plasmid

#53203

Purpose

Expresses HA tagged human ERK2 R67S D321N from puromycin resistance retroviral vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

ERK2 (MAPK1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

R67S/D321N

Promoter

Available since

Sept. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pHR SFFVp BFP-Erk D319N

Plasmid

#102350

Purpose

Lentiviral expressing translational fusion between blue fluorescent protein and Erk2 bearing the D319N mutation

Depositor

Jared Toettcher

Article

Wilson et al Mol Cell. 2017 Sep 7;67(5):757-769.e5. d

Insert

Erk2 (Mapk1 Mouse)

Use

Lentiviral

Tags

BFP

Expression

Mutation

Aspartic acid 319 was mutated to asparagine

Promoter

SFFV

Available since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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