Addgene

We narrowed to 2,255 results for: PRK

Showing: 941 - 960 of 2255 results

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pECE-HA-AMPKbeta1 wt

Plasmid

#31666

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

protein kinase, AMP-activated, beta 1 non-catalytic subunit (PRKAB1 Human)

Use

Tags

HA (YDVPDYASL)

Expression

Mammalian

Mutation

Promoter

Available since

April 11, 2012

Availability

Academic Institutions and Nonprofits only
pENTR-p38KTRmCerulean3

Plasmid

#59149

Purpose

ORF encoding p38 KTR mCerulean3 flanked by Gateway sequences

Depositor

Markus Covert

Article

Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

Insert

p38 Kinase Translocation Reporter (MAPK14 Mouse, Human)

Use

Tags

Expression

Mutation

Promoter

Available since

Sept. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pLentiPGK Puro DEST PKAKTRClover

Plasmid

#59153

Purpose

Lentiviral vector to express PKA KTR mClover under PGK promoter (With Puromycin Resistance)

Depositor

Markus Covert

Article

Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

Insert

PKA (PRKACA Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Sept. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pHR SFFVp BFP-Erk2

Plasmid

#50848

Purpose

Fluorescent reporter for Erk nuclear translocation

Depositor

Wendell Lim

Article

Toettcher et al Cell. 2013 Dec 5;155(6):1422-34. doi: 10

Insert

Erk2 (Mapk1 Mouse)

Use

Lentiviral

Tags

TagBFP

Expression

Mutation

Promoter

Available since

Jan. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag MKK7b1(glu)

Plasmid

#14673

Depositor

Roger Davis

Article

Tournier et al Mol Cell Biol. 1999 Feb . 19(2):1569-81.

Insert

MKK7b1(Glu) (Map2k7 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Ser-269Glu Thr-273Glu

Promoter

Available since

July 25, 2007

Availability

Academic Institutions and Nonprofits only
Parkin

Plasmid

#187897

Purpose

Mammalian cell expression of untagged Parkin

Depositor

Erika Holzbaur

Article

Harding et al Mol Cell. 2023 Sep 7;83(17):3188-3204.e7

Insert

PRKN (PRKN Human)

Use

Tags

None

Expression

Mammalian

Mutation

None

Promoter

CMV

Available since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1 3xFlag-p38alpha

Plasmid

#47575

Purpose

Bacterial expression plasmid for GST-3xFlag-tagged p38alpha

Depositor

Kevin Janes

Article

Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

Insert

p38alpha (Mapk14 Mouse)

Use

Tags

3xFlag and GST

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 28, 2013

Availability

Academic Institutions and Nonprofits only
pENTR-p38KTRClover

Plasmid

#59142

Purpose

ORF encoding p38 KTR mClover flanked by Gateway sequences

Depositor

Markus Covert

Article

Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

Insert

p38 Kinase Translocation Reporter (MAPK14 Mouse, Human)

Use

Tags

Expression

Mutation

Promoter

Available since

Sept. 3, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pGSTag Flag p38 alpha

Plasmid

#20815

Depositor

Roger Davis

Article

Enslen et al J Biol Chem. 1998 Jan 16. 273(3):1741-8.

Insert

pGSTag Flag p38 alpha (Mapk14 Mouse)

Use

Tags

Flag

Expression

Bacterial

Mutation

Promoter

Available since

April 14, 2009

Availability

Academic Institutions and Nonprofits only
pCDNA3 Flag MKK7a1 (glu)

Plasmid

#14540

Depositor

Roger Davis

Article

Tournier et al Proc Natl Acad Sci U S A. 1997 Jul 8. 94

Insert

MKK7a1(Glu) (Map2k7 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Ser-198Glu Thr-202Glu

Promoter

Available since

July 25, 2007

Availability

Academic Institutions and Nonprofits only
pcDNATM3.1/V5-His TOPO-PKCi-K274W

Plasmid

#55690

Purpose

expresses dominant negative human PKCi tagged with V5

Depositor

Pedro Salas

Article

Wald et al J Cell Sci. 2008 Mar 1;121(Pt 5):644-54.

Insert

Homo sapiens protein kinase C, iota (PRKCI) (PRKCI Human)

Use

Tags

V5/His

Expression

Mammalian

Mutation

A120E and K274W

Promoter

CMV

Available since

Aug. 14, 2014

Availability

Academic Institutions and Nonprofits only
pcDNATM3.1/V5-His TOPO-PKCi-A120E

Plasmid

#55687

Purpose

expresses constitutively active human PKCi tagged with V5

Depositor

Pedro Salas

Article

Wald et al J Cell Sci. 2008 Mar 1;121(Pt 5):644-54.

Insert

Homo sapiens protein kinase C, iota (PRKCI) (PRKCI Human)

Use

Tags

V5/His

Expression

Mammalian

Mutation

A120E

Promoter

CMV

Available since

July 28, 2014

Availability

Academic Institutions and Nonprofits only
pCDNA3 Flag MKK7a1 (K76A)

Plasmid

#14541

Depositor

Roger Davis

Article

Tournier et al Proc Natl Acad Sci U S A. 1997 Jul 8. 94

Insert

MKK7a1(K76A) (Map2k7 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Lys-76Ala

Promoter

Available since

July 25, 2007

Availability

Academic Institutions and Nonprofits only
pCDNA3 Flag MKK7a1 (ala)

Plasmid

#14539

Depositor

Roger Davis

Article

Tournier et al Proc Natl Acad Sci U S A. 1997 Jul 8. 94

Insert

MKK7a1(Ala) (Map2k7 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Ser-198Ala Thr-202Ala

Promoter

Available since

July 25, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3-Ro2

Plasmid

#24503

Depositor

Bruce Conklin

Article

Coward et al Proc Natl Acad Sci U S A. 1998 Jan 6;95(

Insert

RASSL Ro2 (OPRK1 Human)

Use

Tags

FLAG and NLS from prolactin

Expression

Mammalian

Mutation

mutations in extracellular loops 2 (see comments …

Promoter

Available since

June 16, 2010

Availability

Academic Institutions and Nonprofits only
pCDNA3 Flag MKK7g1(K165A)

Plasmid

#14675

Depositor

Roger Davis

Article

Tournier et al Mol Cell Biol. 1999 Feb . 19(2):1569-81.

Insert

MKK7g1(K165A) (Map2k7 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Lys-165Ala

Promoter

Available since

July 25, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3-Rog

Plasmid

#24429

Depositor

Bruce Conklin

Article

Scearce-Levie et al BMC Biol. 2005 . 3():3.

Insert

RASSL (OPRK1 Human)

Use

Tags

FLAG and GFP

Expression

Mammalian

Mutation

mutations in extracellular loops 2 (see comments …

Promoter

Available since

June 16, 2010

Availability

Academic Institutions and Nonprofits only

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pENTR-ERKKTRClover

Plasmid

#59138

Purpose

ORF encoding ERK KTR mClover flanked by Gateway sequences

Depositor

Markus Covert

Article

Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

Insert

ERK Kinase Translocation Reporter (MAPK1 Mouse, Human)

Use

Tags

Expression

Mutation

Promoter

Available since

Sept. 3, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pECE HA AMPKa1 WT

Plasmid

#69504

Purpose

Expresses AMPK alpha1 in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

5'-AMP-activated protein kinase catalytic subunit alpha-1 (PRKAA1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

SV40 early promoter

Available since

April 25, 2016

Availability

Academic Institutions and Nonprofits only

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GFP-ZMYND8

Plasmid

#65401

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

ZMYND8 (ZMYND8 Human)

Use

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge