pcDNA3-Ro2
(Plasmid
#24503)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 24503 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5400
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRASSL Ro2
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Alt nameRo2
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Alt nameKOR
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SpeciesH. sapiens (human)
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Mutationmutations in extracellular loops 2 (see comments below) and 3 (E297Q), making it a RASSL.
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Entrez GeneOPRK1 (a.k.a. K-OR-1, KOP, KOR, KOR-1, KOR1, OPRK)
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Tags
/ Fusion Proteins
- FLAG (N terminal on insert)
- NLS from prolactin (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI/XhoI (not destroyed)
- 3′ cloning site XbaI (destroyed during cloning)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Extracellular Loop 2 (EL2) mutations shown in lowercase: aSSVGISAIVLGvTqpRdgavvCtLQFPspswyWDtvt
Prolactin NLS sequence: MDSKGSSQKGSRLLLLLVVSNLLLCQGVVS
For more information about this plasmid see: http://openwetware.org/wiki/R02-ORF
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3-Ro2 was a gift from Bruce Conklin (Addgene plasmid # 24503 ; http://n2t.net/addgene:24503 ; RRID:Addgene_24503) -
For your References section:
Controlling signaling with a specifically designed Gi-coupled receptor. Coward P, Wada HG, Falk MS, Chan SD, Meng F, Akil H, Conklin BR.. Proc Natl Acad Sci U S A. 1998 Jan 6;95(1):352-7. 10.1073/pnas.95.1.352 PubMed 9419379