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Addgene

pcDNA3-Ro2
(Plasmid #24503)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 24503 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RASSL Ro2
  • Alt name
    Ro2
  • Alt name
    KOR
  • Species
    H. sapiens (human)
  • Mutation
    mutations in extracellular loops 2 (see comments below) and 3 (E297Q), making it a RASSL.
  • Entrez Gene
    OPRK1 (a.k.a. K-OR-1, KOP, KOR, KOR-1, KOR1, OPRK)
  • Tags / Fusion Proteins
    • FLAG (N terminal on insert)
    • NLS from prolactin (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI/XhoI (not destroyed)
  • 3′ cloning site XbaI (destroyed during cloning)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Extracellular Loop 2 (EL2) mutations shown in lowercase: aSSVGISAIVLGvTqpRdgavvCtLQFPspswyWDtvt

Prolactin NLS sequence: MDSKGSSQKGSRLLLLLVVSNLLLCQGVVS

For more information about this plasmid see: http://openwetware.org/wiki/R02-ORF

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-Ro2 was a gift from Bruce Conklin (Addgene plasmid # 24503 ; http://n2t.net/addgene:24503 ; RRID:Addgene_24503)
  • For your References section:

    Controlling signaling with a specifically designed Gi-coupled receptor. Coward P, Wada HG, Falk MS, Chan SD, Meng F, Akil H, Conklin BR.. Proc Natl Acad Sci U S A. 1998 Jan 6;95(1):352-7. 10.1073/pnas.95.1.352 PubMed 9419379