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Addgene

pcDNA3-Rog
(Plasmid #24429)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 24429 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RASSL
  • Alt name
    R03
  • Alt name
    KOR
  • Species
    H. sapiens (human)
  • Mutation
    mutations in extracellular loops 2 (see comments below) and 3 (E297Q), making it a RASSL.
  • Entrez Gene
    OPRK1 (a.k.a. K-OR-1, KOP, KOR, KOR-1, KOR1, OPRK)
  • Tags / Fusion Proteins
    • GFP (N terminal on insert)
    • FLAG (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Extracellular Loop 2 (EL2) mutations shown in lowercase: aSSVGISAIVLGvTqpRdgavvCtLQFPspswyWDtvt

For more information about this construct see: http://openwetware.org/wiki/R03:GFP-tagged_RASSL

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-Rog was a gift from Bruce Conklin (Addgene plasmid # 24429 ; http://n2t.net/addgene:24429 ; RRID:Addgene_24429)
  • For your References section:

    Engineered G protein coupled receptors reveal independent regulation of internalization, desensitization and acute signaling. Scearce-Levie K, Lieberman MD, Elliott HH, Conklin BR. BMC Biol. 2005 . 3():3. 10.1186/1741-7007-3-3 PubMed 15707483