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pLVX-puro_G-CEPIA1er
Plasmid#164590PurposeStable integration and expression of G-CEPIA1er in mammalian cellsDepositorInsertG-CEPIA1er
UseLentiviralTagsMycExpressionMammalianMutationG-GECO1.1 E299D M304L D328N N345K F360W G369S E37…PromoterCMVAvailable sinceFeb. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSLIK-Neo
Plasmid#25735Purpose3rd generation Lentiviral vector for Tet-based inducible shRNA or cDNA expression, constitutive Neomycin resistance gene coexpression.DepositorTypeEmpty backboneUseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceJuly 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-MCU-V5-HIS
Plasmid#31731DepositorInsertMCU (MCU Human)
UseTagsV5-HIS6XExpressionMammalianMutationPromoterCMVAvailable sinceAug. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCMV-3XFLAG-hINO80
Plasmid#44149DepositorInserthINO80 full length (INO80 Human)
UseTags3xFlagExpressionMammalianMutationPromoterCMVAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
TFORF2938
Plasmid#144414PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertSOX5 (SOX5 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
STARR-seq luciferase validation vector_ORI_neg.cont
Plasmid#99315PurposeNegative control for luciferase validation vectorsDepositorInsertneutral region; chr3: 55198740-55200041
UseLuciferase; Starr-seq luciferase validation vectorTagsExpressionMammalianMutationPromoterAvailable sinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV_BiPVe4_eGFP
Plasmid#221463PurposeAAV construct expressing eGFP driven by chandelier cell-targeting enhancer. Alias: pAAV_WDC0004_eGFPDepositorInserteGFP
UseAAVTagsExpressionMutationPromoterAvailable sinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENN.AAV.CamKII.GCaMP6f.WPRE.SV40 (AAV9)
Viral Prep#100834-AAV9PurposeReady-to-use AAV9 particles produced from pENN.AAV.CamKII.GCaMP6f.WPRE.SV40 (#100834). In addition to the viral particles, you will also receive purified pENN.AAV.CamKII.GCaMP6f.WPRE.SV40 plasmid DNA. CamKII-driven GCaMP6f calcium sensor. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCaMKIITagsNoneAvailable sinceApril 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
MP1
Plasmid#69627PurposeUntargetted Bacterial MutagenesisDepositorInsertdnaQ926 umuD' umuC recA730
UseTagsExpressionBacterialMutationPromoterE. coli PBADAvailable sinceSept. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hDlx-GqDREADD-dTomato-Fishell-4 (AAV9)
Viral Prep#83897-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-hDlx-GqDREADD-dTomato-Fishell-4 (#83897). In addition to the viral particles, you will also receive purified pAAV-hDlx-GqDREADD-dTomato-Fishell-4 plasmid DNA. hDlx-driven expression of GqDREADD (hM3D) and nuclear localized dTomato (physically separate) for activation of interneurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterhDlxTagsdTomatoAvailable sinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE
Plasmid#51509PurposeCan be used to generate AAV virus that will express cytoplasmic tdTomato and presynaptic (synaptophysin-fused) EGFP in the presence of Cre in neurons from the synapsin promoterDepositorHas ServiceAAV1InserttdTomato-T2A-SypEGFP
UseAAVTagsExpressionMutationPromoterphSyn1Available sinceMay 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCF159_BRL
Plasmid#225962PurposeCMV-Intron-BRL (env protein). Expresses BRL (BaEVRLess) env for VLP production.DepositorInsertCMV-Intron-BRL (env protein)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAX APP Swe/Ind
Plasmid#30145DepositorInserthuman APP 695 Swedish/Indiana mutation (APP Human)
UseTagsExpressionMammalianMutationSwedish and Indiana; K595N, M596L and V642FPromoterAvailable sinceAug. 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDuBir-Lbu-dCas13a-avitag
Plasmid#100817PurposeDual expression of Lbu-dCas13a with a C-terminal avi-tag and BirA to biotinylate Lbu-dCas13aDepositorInsertLbu_Cas13a_R472A_H477A_R1048A_H1053A
UseTagsAviTag and His6-MBPExpressionBacterialMutationR472A, H477A, R1048A, H1053APromoterT7Available sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 hygro
Plasmid#24150Purpose3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses hygromycin for selection.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral and RNAiTagsExpressionMammalianMutationPromoterU6 for shRNAAvailable sinceMarch 15, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag p38 alpha
Plasmid#20351DepositorInsertpDCNA3 Flag p38 alpha (Mapk14 Mouse)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceApril 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-GFP-LC3-RFP-LC3ΔG
Plasmid#168997PurposeExpresses GFP-LC3-RFP-LC3ΔG in mammalian cells and zebrafish to measure autophagic fluxDepositorInsertmicrotubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
UseTagsEGFP and mRFP1ExpressionMammalianMutationPromoterAvailable sinceApril 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPPED
Plasmid#162468PurposeDestination Expression vector for dicot plant prime editingDepositorInsertsCas9H840A
attR gateway destination cassette
UseTagsMLV reverse transcriptaseExpressionPlantMutationChange histidine 840 to Alanine based sequence o…Promoter2x35SAvailable sinceDec. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-fDIO-GCaMP6f (AAV1)
Viral Prep#128315-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-Ef1a-fDIO-GCaMP6f (#128315). In addition to the viral particles, you will also receive purified pAAV-Ef1a-fDIO-GCaMP6f plasmid DNA. EF1a-driven, Flp-dependent expression of calcium sensor GCaMP6f. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsNoneAvailable sinceMarch 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
spNW30
Plasmid#173485PurposeExpresses spNW30 in Bl21DepositorInsertspNW30
UseTagsHistagExpressionBacterialMutationPromoterAvailable sinceSept. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA_RFP-TEVprotease
Plasmid#124947PurposeExpression of RFP-TEVproteaseDepositorInsertRFP-TEVprotease
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
AmCyan1-N1
Plasmid#54616PurposeLocalization: N1 Cloning Vector, Excitation: 453, Emission: 486DepositorTypeEmpty backboneUseTagsAmCyan1ExpressionMammalianMutationPromoterAvailable sinceJuly 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLVX-NLS-HA-TurboID-PCNA
Plasmid#215076PurposeExpresses NLS-HA-TurboID-PCNA in mammalian cells from a lentiviral vector.DepositorInsertPCNA (PCNA Human)
UseLentiviralTagsNLS-HA-TurboIDExpressionMammalianMutationPromoterCMVAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_HaloTag9_SKL
Plasmid#175542PurposePeroxysomal expression of HaloTag9 in mammalian cellsDepositorInsertHaloTag9-SKL
UseTagsExpressionMammalianMutationHaloTag9 = HaloTag7-Q165H-P174RPromoterAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE2-P2A-GFP
Plasmid#132776PurposePrime editing in mammalian cells with co-translational GFP expressionDepositorInsertPE2-P2A-GFP
UseTagsSV40 NLSExpressionMammalianMutationSee manuscriptPromoterCMVAvailable sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMIH-TOMM20-Halo
Plasmid#111626PurposeFusion protein used to visualise mitochondria upon the addition of Halo specific dye, has hygromycin selectionDepositorInsertTOMM20 (Tomm20 Mouse)
UseRetroviralTagsHaloTagExpressionMammalianMutationPromoterMSCVAvailable sinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
phGluc
Plasmid#22522DepositorInsertGaussia luciferase
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 7, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-hChR2(H134R)-mCherry
Plasmid#26976PurposeAAV expression of humanized ChR2 with H134R mutation fused to mCherry, for optogenetic excitationDepositorHas ServiceAAV5, AAV8, and AAV9InserthChR2(H134R)
UseAAVTagsmCherryExpressionMammalianMutationH134RPromoterhSynAvailable sinceFeb. 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
MBP-Benzonase
Plasmid#218192PurposeBacterial expression of a periplasmically expressed MBP-tagged Serratia Marcescens NucA (Benzonase); also known as c001DepositorInsertnucA
UseTagsMBPExpressionBacterialMutationPromoterAvailable sinceApril 24, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEGFP Kindlin2
Plasmid#105305PurposeTo study effect of Kindlin 2 on Cell/ECM adhesionDepositorInsertKindlin2 (FERMT2 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-miRFP680-P2A-GFP
Plasmid#197164PurposeExpresses the protein of miRFP680-P2A-GFP in mammalian cellsDepositorInsertmiRFP680-P2A-GFP
UseAAVTagsExpressionMutationPromoterAvailable sinceMarch 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSLQ10878
Plasmid#183964PurposeCAG-hyperCas12a(nuclease active)-HA-P2A-mCherry-T2A-HygroRDepositorInsertHyperdCas12a
UseCRISPRTagsHA, Hygromycin Resistance, and mCherryExpressionMammalianMutationD156R, E292R, D235R, D350RPromoterCAGAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-hChR2(H134R)-EYFP (AAV5)
Viral Prep#26973-AAV5PurposeReady-to-use AAV5 particles produced from pAAV-hSyn-hChR2(H134R)-EYFP (#26973). In addition to the viral particles, you will also receive purified pAAV-hSyn-hChR2(H134R)-EYFP plasmid DNA. hSyn-driven, humanized channelrhodopsin H134R mutant fused to EYFP for optogenetic activation. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsEYFPAvailable sinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
T4 td upstream intron-PABP
Plasmid#189972PurposePart 1 plasmid to introduce the T4 td upstream intron and PABP spacer into circRNAsDepositorInsertT4 td upstream intron-PABP
UseCloningTagsExpressionMutationPromoterN/AAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSIN-PAmCherry-mSNCA-NE
Plasmid#102364PurposeLentiviral overexpression of PA-mCherry synuclein alpha fusionDepositorInsertsUseLentiviralTagsNEExpressionMammalianMutationPromoterEF-1aAvailable sinceApril 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
HBA1 full 3'UTR-T4 td downstream intron
Plasmid#189974PurposePart 6 plasmid to introduce the HBA1 full 3'UTR and T4 td downstream intron into circRNAsDepositorInsertHBA1 full 3'UTR-T4 td downstream intron
UseCloningTagsExpressionMutationPromoterN/AAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
CIDAR MoClo Extension, Volume I
Plasmid Kit#1000000161PurposeThis kit contains 110 plasmids designed to quickly and efficiently build a wide variety of gene expression constructs using the CIDAR-standard MoClo system.DepositorAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLARRYv2-EGFP-T2A-Flpo
Plasmid#233212PurposeCloning expressed barcodes at the EcoRV site for preparing lentiviral libaries for single-cell lineage tracing experiments, with EGFP-T2A-Flpo for selectionDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
AbVec_IGHC
Plasmid#183701PurposeEncode human IgG1 heavy chain constant region. For cloning variable region and mAb expression.DepositorInsertIGHG1 (IGHG1 Human)
UseTagsExpressionMammalianMutationPromoterSP6Available sinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ÂRAGE
Plasmid#71435Purposemammalian expression of human RAGEDepositorInsertRAGE (AGER Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMXs-c-Myc-T58A
Plasmid#13372PurposeRetroviral expression of mouse c-Myc T58A mutantDepositorInsertmyelocytomatosis oncogene (T58A mutant) (Myc Mouse)
UseRetroviralTagsExpressionMammalianMutationTyrosine 58 to AlaninePromoterAvailable sinceJan. 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
AbVec_IGKC
Plasmid#183702PurposeEncode human Ig kappa chain constant region. For cloning variable region and mAb expression.DepositorInsertIGKC (IGKC )
UseTagsExpressionMammalianMutationPromoterSP6Available sinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJFRC81-10XUAS-IVS-Syn21-GFP-p10
Plasmid#36432DepositorInsertGFP
UseTagsExpressionInsectMutationPromoterAvailable sinceMay 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-mScarlet (AAV1)
Viral Prep#131001-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-hSyn-mScarlet (#131001). In addition to the viral particles, you will also receive purified pAAV-hSyn-mScarlet plasmid DNA. Synapsin-driven mScarlet control vector. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsmScarletAvailable sinceNov. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
TFORF0989
Plasmid#141780PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertKLF9 (KLF9 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKII-GRAB_g5-HT3.0
Plasmid#208723PurposeExpresses the green 5-HT sensor GRAB_g5-HT3.0 in excitatory neuronsDepositorInsertGreen fluorescent 5-HT sensor GRAB_g5-HT3.0
UseAAVTagsExpressionMutationPromoterCaMKIIAvailable sinceOct. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJT260 Lenti pEF-dCas9-3XFLAG-VPR-T2A-tagBFP-P2A-Blast
Plasmid#187336PurposedCas9 effector fusion for manipulating transcriptionDepositorInsertdCas9-3XFLAG-VPR-T2A-tagBFP-P2A-Blast
UseCRISPR and LentiviralTags3XFLAGExpressionMammalianMutationPromoterpEF1aAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSin Helper
Plasmid#127694PurposePlasmid contains the Sindbis glycoprotein genes and can be converted to mRNA for initiating Sindbis virus packaging. See Resource Information section for annotated plasmid sequence.DepositorInsertSindbis Glycoproteins
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 3, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
TFORF0985
Plasmid#142249PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertKLF4 (KLF4 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 23, 2023AvailabilityAcademic Institutions and Nonprofits only
