AbVec_IGHC
(Plasmid
#183701)
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PurposeEncode human IgG1 heavy chain constant region. For cloning variable region and mAb expression.
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 183701 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneAbVec
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Backbone manufactureradapted from Burton lab
- Backbone size w/o insert (bp) 5000
- Total vector size (bp) 6500
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameIGHG1
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Alt nameIgG1 heavy chain
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SpeciesH. sapiens (human)
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Insert Size (bp)1000
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GenBank ID3500
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Entrez GeneIGHG1
- Promoter SP6
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer SP6-F
- 3′ sequencing primer SV40-R (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byadapted from Burton lab mAb expression plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AbVec_IGHC was a gift from David Nemazee (Addgene plasmid # 183701 ; http://n2t.net/addgene:183701 ; RRID:Addgene_183701)