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A viral strategy for targeting and manipulating interneurons across vertebrate species.

Dimidschstein J, Chen Q, Tremblay R, Rogers SL, Saldi GA, Guo L, Xu Q, Liu R, Lu C, Chu J, Avery MC, Rashid MS, Baek M, Jacob AL, Smith GB, Wilson DE, Kosche G, Kruglikov I, Rusielewicz T, Kotak VC, Mowery TM, Anderson SA, Callaway EM, Dasen JS, Fitzpatrick D, Fossati V, Long MA, Noggle S, Reynolds JH, Sanes DH, Rudy B, Feng G, Fishell G
Nat Neurosci. 2016 Oct 31. doi: 10.1038/nn.4430. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
83894pAAV-hDlx-Flex-dTomato-Fishell_7Cre recombinase-dependent dTomato expression in forebrain GABA-ergic interneurons under the control of the hDlx enhancer element
83895pAAV-hDlx-Flex-GFP-Fishell_6Cre recombinase-dependent GFP expression in forebrain GABA-ergic interneurons under the control of the hDlx enhancer element
83896pAAV-hDlx-GiDREADD-dTomato-Fishell-5Gi-DREADD (P2A) nuclear dTomato expression in forebrain GABA-ergic interneurons under the control of the hDlx enhancer element
83897pAAV-hDlx-GqDREADD-dTomato-Fishell-4Gq-DREADD (P2A) nuclear dTomato expression in forebrain GABA-ergic interneurons under the control of the hDlx enhancer element
83898pAAV-mDlx-ChR2-mCherry-Fishell-3ChR2-mCherry fusion expression in forebrain GABA-ergic interneurons under the control of the mDlx enhancer element
83899pAAV-mDlx-GCaMP6f-Fishell-2GCaMP6f expression in forebrain GABA-ergic interneurons under the control of the mDlx enhancer element
83900pAAV-mDlx-GFP-Fishell-1GFP expression in forebrain GABA-ergic interneurons under the control of the mDlx enhancer element

Antibodies from Article