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Plasmid#173926PurposeGateway entry clone (attL5 & attL2) for Cas12j2 crRNA expression under ZmUbi promoter with ribozyme processingDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only
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pSB167 - pL2_pSB90_2x35S::fLUC-I::tNOS_tMAS::rLUC-I::pMAS
Plasmid#123200Purposebinary plant vector for transient expression of a Renilla luciferase (rLUC, with intron) and a firefly luciferase (fLUC, with intron) in plantsDepositorInsert2x35S::fLUC-I::tNOS tMAS::rLUC-I::pMAS
UseLuciferaseTagsExpressionPlantMutationPromoterAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-VPS35
Plasmid#21689DepositorInsertVacuolar Protein Sorting 35 homolog (VPS35 Human)
UseGateway entry cloneTagsExpressionMutationlast nucleotide of stop codon removed for c-termi…PromoterAvailable sinceApril 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pSW725 - pUFO>>
Plasmid#116000Purposedestination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expressionDepositorInsertpUFO:GR-LhG4:tRBCS:F-H adapter-H-A adapter::pOp6:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
UseTagsHDEL and SP(ER)ExpressionPlantMutationPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLKO-shSmad2
Plasmid#37051DepositorInsertSmad2 (SMAD2 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterU6Available sinceJan. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEL033
Plasmid#137902PurposeSTU2.0 rAPOBEC1-SpyCas9(D10A) for plant genome base editingDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceFeb. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
cfmTurquoise-N
Plasmid#139284PurposeCys-free mTurquoise (improved ECFP) for extracellular FRET analysis. This variant contains the same set of mutations (C48S and C70M ) as cfSGFP2. Molecular brightness is similar to mTurquoise.DepositorInsertcfmTurquoise
UseTagsMulti-cloning sitesExpressionMammalianMutationCys48Ser and Cys70/MetPromoterAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 c-SRC (250-536) K298M HA
Plasmid#42210DepositorInsertc-SRC (250-536) HA (SRC Human)
UseTagsHAExpressionMammalianMutationcontains amino acids 250-536; K298MPromoterCMVAvailable sinceJan. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-HLA-B57
Plasmid#135516PurposeMammalian expression of myc-tagged HLA-B*57:01DepositorInsertHLA-B*57:1 (HLA-B Human)
UseTagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDECKO_mCherry_non-targeting
Plasmid#140109PurposeCRISPR-Cas9 library validation (negative control)DepositorInsertgRNA1+scaffold+H1promoter+gRNA2
UseLentiviralTagsExpressionMutationPromotergRNA1 under U6 and gRNA2 under H1Available sinceApril 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Ost4-GFP-MTn
Plasmid#154351PurposeMammalian expression of MTnDepositorInsertMTn
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P1-hPXR-LBD
Plasmid#67769PurposeExpression GST-hPXR-LBD in bacteriaDepositorInserthuman pregnane X receptor (PXR) ligand binding domain (NR1I2 Human)
UseTagsGSTExpressionBacterialMutationContains amino acids 107 to 434 of human PXRPromoterTacAvailable sinceJuly 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pICE-HA-Ku70-siR-WT
Plasmid#82329PurposePlasmid for constitutive or doxycycline-inducible expression of wild-type human Ku70 resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.DepositorInsertKu70 (XRCC6 Human)
UseTagsHAExpressionMammalianMutationPromoterCMV-tetAvailable sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1P POLD1_2
Plasmid#160792PurposeSuppress POLD1DepositorInsertshPOLD1_2
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCI-Neo-PTPRT (WT)
Plasmid#16630DepositorInsertPTPRT (PTPRT Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-V5-Lem2
Plasmid#120245PurposeExpresses V5-tagged Lem2 in lentiviral vectorDepositorInsertLem2 (Lemd2 Mouse)
UseLentiviralTagsV5ExpressionMutationPromoterEF1aAvailable sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYPQCas12j2
Plasmid#189780PurposeGateway entry plasmid (attL1 & attR5) expressing NLS-Cas12j2-NLS, without promoterDepositorInsertCas12j2
UseCRISPR; Gateway compatible cas12j2 entry cloneTagsNLSExpressionPlantMutationPromoterAvailable sinceOct. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-mGold-MannII-N-10
Plasmid#158005PurposeVisualization of Golgi in mammalian cells using mGoldDepositorInsertMannII (Man2a1 Mouse)
UseTagsmGoldExpressionMammalianMutationInsert contains only aa 1-112 of MannIIPromoterCMVAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ-Cas12j2
Plasmid#173924PurposeGateway entry plasmid (attL1 & attR5) expressing NLS-Cas12j2-NLSDepositorInsertNLS-Cas12j2-NLS
UseCRISPRTagsNLSExpressionPlantMutationPromoterAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Stop-EGFP (MSE)
Plasmid#154469PurposeMSCV-Stop-EGFP-pgk-Neo (retroviral construct containing the RFP-based translational stop cassette)DepositorTypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only