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Viral Prep#137122-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-Ef1a-Con/Fon-GCaMP6F (#137122). In addition to the viral particles, you will also receive purified pAAV-Ef1a-Con/Fon-GCaMP6F plasmid DNA. EF1a-driven, Cre and Flp-dependent expression of GCaMP6f. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsNoneAvailable sinceMarch 12, 2021AvailabilityAcademic Institutions and Nonprofits only
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pET20b-NLuc-MBP-6xHis
Plasmid#135931PurposeExpresses NanoLuciferase in bacterial cells.DepositorInsertNanoLuciferase
UseTagsMBP-6xHisExpressionBacterialMutationPromoterT7Available sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-3'TwStrep
Plasmid#208618PurposeEmpty vector for mammalian expression, insert Twin Strep tag using BamHI and AflII site of pcDNA3.1 (-), both site retained, BamHI in-frame with Twin Strep tagDepositorTypeEmpty backboneUseTagsTwin StrepExpressionMammalianMutationPromoterCMVAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
peGFP C3-SPG20
Plasmid#218578PurposeExpresses GFP-tagged human SPG20 in mammalian cellsDepositorInsertSPG20 (SPART Human)
UseTagseGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCC_05 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dCas9-NLS-VPR-2A-Puro-WPRE
Plasmid#139090PurposeExpresses human codon-optimized inactive SpCas9 fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.DepositorInsertdSpCas9-VPR
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A, H840APromoterAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
mClY-N1
Plasmid#90457PurposeA YFP-based chloride sensor with enhanced chloride sensitivity, photostability and reduced pH interference. The sequence of mClY is EYFP-F46L/Q69K/H148Q/I152L/V163S/S175G/S205V/A206K.DepositorInsertmClY-N1
UseTagsExpressionMammalianMutationEYFP-F46L/Q69K/H148Q/I152L/V163S/S175G/S205V/A206…PromoterAvailable sinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
moxGFP
Plasmid#68070PurposeC-terminal fusion to moxGFPDepositorTypeEmpty backboneUseTagsmoxGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pORI28
Plasmid#71595PurposeEC1000 containing pORI28. Used as the source of plasmid pORI28. With pTRK669, part of a system for chromosomal integration in gram positive bacteria.DepositorTypeEmpty backboneUseIntegration vector for bacteriaTagsExpressionMutationPromoterAvailable sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBb(RK2)1k-GFPuv
Plasmid#106394PurposeIPTG-inducible trc promoter expressing gfpuv on RK2 originDepositorInsertGFPuv
UseTagsExpressionBacterialMutationPromotertrcAvailable sinceMarch 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
p23-NES-RfxCas13d-msfGFP-NES-Flag
Plasmid#165076Purposeoverexpression of RfxCas13d in human cellsDepositorInsertRfxCas13d
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKM464 (deletions)
Plasmid#108322PurposeOrbit integrating plasmid to delete target gene.DepositorInsertBxb1 attB site
UseOrbit integration plasmid for creating knockouts …TagsExpressionMutationPromoterAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pgRNA-humanized
Plasmid#44248PurposeExpression of customizable guide RNA (gRNA) under cotnrol of murine U6 promoter, also contains a CMV-puro-t2A-mCherry expression cassette for mammalian gene knockdownDepositorInsertguide RNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermouse U6Available sinceApril 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLJC6-2XFLAG-TMEM192
Plasmid#104435PurposeExpresses 2XFLAG-TMEM192 in mammalian cellsDepositorInsertTMEM-192 (TMEM192 Human)
UseLentiviralTags2XFLAGExpressionMammalianMutationPromoterUbiquitinAvailable sinceJuly 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
FLHKI-pGFPN3
Plasmid#21917DepositorInsertFull length human HKI in pGFP-N3 (HK1 Human)
UseTagsGFPExpressionMammalianMutationThis is full length human HKI in pGFP-N3PromoterAvailable sinceSept. 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
Beta-2-adrenergic receptor-mRFP
Plasmid#67145PurposeBeta-2-adrenergic receptor tagged with mRFP on carboxyl terminusDepositorInsertBeta-2-adrenergic receptor-mRFP (ADRB2 Human, Discosoma sp.)
UseTagsmRFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
AbVec2.0-mIghg2b
Plasmid#127155PurposeExpression of secretory immunoglobulin heavy chains in mammalian cells, mouse (C57BL/6), IgG2b isotypeDepositorInsertIghg2b (Ighg2b Mouse)
UseTagsExpressionMammalianMutationPromoterhCMV IE1 gene promoterAvailable sinceNov. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Bsd
Plasmid#214370PurposeCloning vector with BlasticidinDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
mCherry-PLK1-N-16
Plasmid#55119PurposeLocalization: Centrosomes, Excitation: 587, Emission: 610DepositorInsertPLK1 (PLK1 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-puro-TagBFP-β-ACTIN-3′UTR-24xMS2
Plasmid#205161Purposeexpress β-ACTIN-3′UTR-24xMS2DepositorInsertβ-ACTIN-3′UTR-24xMS2
UseTagstagBFPExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only