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Plasmid#124121PurposeBacterial expression plasmid for PURE system componentsDepositorInsertTyrosyl-tRNA ligase (tyrS Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
As0
Plasmid#123158PurposeArsenic sensor used in the sensor cell array. pSB4A3 carrying J109-30arsR-t-ParsR-ABS67-30hrpR-30hrpS-t-PhrpLE-30RinA-t-PrinA-30gfp-tDepositorInsertJ109-30arsR-t-ParsR-ABS67-30hrpR-30hrpS-t-PhrpLE-30RinA-t-PrinA-30gfp-t
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ109, ParsR-ABS67, PhrpLE, PrinAAvailable sinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
FUCas9Cherry
Plasmid#70182PurposeExpresses Cas9 with fluorescent Cherry reporterDepositorInserthumanised S.pyogenes cas9
UseCRISPR and LentiviralTags3xFLAGExpressionMammalianMutationPromoterhUbCAvailable sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-ChR2(H134R)-GFP
Plasmid#58880PurposeAAV expression of ChR2(H134R)-GFP under the Syn promoterDepositorHas ServiceAAV Retrograde and AAV8InsertChR2(H134R)-GFP
UseAAVTagsGFPExpressionMammalianMutationH134RPromoterSynAvailable sinceAug. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PEmax-P2A-hP53DD-P2A-hMLH1dn
Plasmid#214102PurposeMammalian expression of SpCas9 PEmax prime editor with P2A-human P53DD and P2A-human MLH1dnDepositorInsertPE-Plus
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEB2-E2-Crimson
Plasmid#104010PurposePlasmid encoding E2-CrimsonDepositorInsertE2-Crimson
UseLow copyTagsExpressionBacterialMutationMutations relative to DsRed (R2D, S4T, K5E, E10P,…PromoterproCAvailable sinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
GFP-P4M-SidM
Plasmid#51469PurposeBiosensor for PtdIns4PDepositorInsertP4M-SidM(546-647)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
SARS-CoV2 Nucleocapsid
Plasmid#153201PurposeExpresses myc-tagged nucleocapsid (N) from SARS-CoV2 in mammalian cellsDepositorInsertNucleocapsid from SARS-CoV-2 (N severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2))
UseTagsMyc tag (5X)ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 15, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCDH-EF1-Luc2-P2A-copGFP
Plasmid#72485PurposeExpress Luc2 and copGFPDepositorInsertLuc2-P2A-copGFP
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1Available sinceJan. 11, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLVX-Puro-hGSDME
Plasmid#154876PurposeExpresses hGSDME in mammalian cellsDepositorInsertGSDME (GSDME Human)
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHiUGE HA-epitope donor ORF1
Plasmid#200372PurposeTagging endogenously expressed proteins with HA epitope, C-terminalDepositorInsertHA epitope
UseTagsExpressionMammalianMutationNAPromoterAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
FURIN-bio-His
Plasmid#51755PurposeExpresses full-length Furin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertFURIN (FURIN Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBABE hBRM
Plasmid#1961DepositorInsertBRM (SMARCA2 Human)
UseRetroviralTagsflagExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSyn.iGluSnFR3.v857.PDGFR
Plasmid#178329PurposeFluorescent reporter for glutamate, third generation, variant 857. iGluSnFR3.v857DepositorHas ServiceAAV1InsertpAAV hSyn iGluSnFR3 v857.PDGFR
UseAAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…TagsIgK-chain, Myc epi tag, and PDGFR TM DomainExpressionMammalianMutationPromoterAvailable sinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNL-CEF-BAFFR-CFP
Plasmid#187002PurposeExpression of human BAFFR-CFP fusion proteinDepositorInsertBAFFR (TNFRSF13C Human)
UseLentiviralTagsCFPExpressionMutationPromoterAvailable sinceJuly 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
ACPPiso2-V5
Plasmid#113053PurposeExpresses human prostatic acid phosphatase (isoform 2) in mammalian cellsDepositorInsertprostatic acid phosphatase isoform 2 (ACP3 Human)
UseTagsV5ExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTdTomato-L5
Plasmid#140994PurposetdTomato, StrepR, L5int, attP to create RFP mycobacteriaDepositorInserttdTomato
UseTagsExpressionBacterialMutationPromoterhsp60Available sinceOct. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHIV-Luciferase
Plasmid#21375Purposeself-inactivating 3rd generation lentiviral plasmid for co-expression of your gene of interest and LuciferaseDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 19, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MSP1D1delltaH5
Plasmid#71714PurposeMembrane scaffold protein (MSP), helix 5 deletionDepositorInsertApolipoprotein A1 (APOA1 Human)
UseTags6xHis and TEV siteExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPY22-mNeonGreen-Hyg
Plasmid#137082PurposeCodon-optimized mNeonGreen for expression in Arabidopsis thaliana and Physcomitrella patensDepositorInsertmNeonGreen-Flag
UseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only