-
Plasmid#50590PurposeCRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sitesDepositorInsertT1, U626t plus, U629p, T2
UseCRISPR; Pcr templateTagsExpressionPlantMutationPromoterAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
Cas9-GFP_sg_mAMPKa1
Plasmid#79004PurposeExpresses WT Cas9 and GFP, along with a sgRNA to mouse AMPK alpha 1.DepositorInsertPrkaa1 (Prkaa1 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJune 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDGE6
Plasmid#79476PurposesgRNA shuttle vector; module 1EDepositorInsertpAtU6-ccdB-sgRNA (ccdB(letD) )
UseCRISPR and Synthetic BiologyTagsExpressionMutationBsaI site eliminatedPromoterAvailable sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJYS2cm_crtYf
Plasmid#85543PurposeConstitutive transcription of crRNA of crtYf, Chloramphenicol resistanceDepositorInsertcrRNA of crtYf
UseCRISPR; Shuttle vector corynebacterium glutamicum…TagsExpressionBacterialMutationPromoterAvailable sinceMay 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSAG1::CAS9-U6::sgUPRT
Plasmid#54467PurposeEncodes CAS9 nuclease (GFP fusion) under control of the Toxoplasma gondii SAG1 promotor. Vector also provides U6 controlled expression of a single guide RNA for disruption of genes by CRISPR.DepositorInsertSAG1 5' UTR
UseCRISPR; Toxoplasma expressionTagsExpressionMutationPromoterAvailable sinceJune 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGuide
Plasmid#64711PurposeCloning and expression of Streptococcus pyogenes guide RNADepositorInsertStreptococcus pyogenes guide RNA
UseCRISPRTagsExpressionMammalianMutationPromoterhU6Available sinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEn-Chimera
Plasmid#61432PurposeGateway-Entry vector containing AtU6-26 promoter and sgRNA backboneDepositorInsertAtU6-26:sgRNA
UseCRISPRTagsExpressionPlantMutationPromoterAtU6-26Available sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPEG
Plasmid#162466PurposeEntry vector for gRNA insertion in plant prime editing expression vectorDepositorTypeEmpty backboneUseEntry vector for grnaTagsExpressionMutationPromoterCmYLCVAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHEE901
Plasmid#91707PurposeCRISPR/Cas9-mediated base editing in Arabidopsis. Contains Cas9(D10A) and empty gRNA scaffold, HygRDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRN227
Plasmid#127222PurposeBeYDV replicon with 35S::Cas9, WUS2 and AtSTM, sgRNA targeting PDSDepositorInsertCas9, WUS2, AtSTM, sgRNA
UseTagsExpressionPlantMutationcodon optimized CDSsPromoterAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide_CjCas9-Puro
Plasmid#202564PurposehU6-BsmBI-gRNA-PuroDepositorInsertCjCas9 sgRNA cassette
UseTagsExpressionMammalianMutationPromoterhU6Available sinceSept. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEP163
Plasmid#137881PurposePiggyBac donor vector for expression of mRFP-T2A-BlasticidinRDepositorInsertmRFP-T2A-BlasticidinR
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMM114
Plasmid#127211PurposeBeYDV replicon with constitutive Luc expression, WUS2 and IPT, sgRNA targeting PDSDepositorInsertWUS2, IPT, sgRNA, Luc
UseTagsExpressionPlantMutationcodon optimized CDSsPromoterAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRGE32
Plasmid#63159PurposeExpress sgRNA/PTG with rice snoRNA U3 promoter and Cas9 with rice ubiquitin promoter for transient expression in protoplastDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterRice snoRNA U3 promoter for gRNA/PTG expression a…Available sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHSE901
Plasmid#91708PurposeCRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, HygRDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEM-T Easy-AtpU6-26
Plasmid#160218PurposeAtpU6-26 Golden Gate level 0 piece to express gRNAsDepositorInsertpAtU6-26 promoter
UseGolden gate level 0 piece to express grnasTagsExpressionMutationPromoterAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ141B2.0
Plasmid#99897PurposeExpress single gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAtU3Available sinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pXPR_501
Plasmid#96922Purposefor CRISPRa, lentiviral expression of gRNA scaffold using activator VP64DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBWB419
Plasmid#140637PurposeRSF1010-Cas9-pcaHG gRNA. Vector with constitutive Cas9 with gRNA targeting pcaHG locus.DepositorInsertCas9
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMM113
Plasmid#127210PurposeBeYDV replicon with constitutive Luc expression, WUS2 and AtSTM, sgRNA targeting PDSDepositorInsertWUS2, AtSTM, sgRNA, Luc
UseTagsExpressionPlantMutationcodon optimized CDSsPromoterAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only