Generation of chromosomal deletions in dicotyledonous plants employing a user-friendly genome editing toolkit.
Ordon J, Gantner J, Kemna J, Schwalgun L, Reschke M, Streubel J, Boch J, Stuttmann J
Plant J. 2016 Aug 31. doi: 10.1111/tpj.13319. PubMed Article
Ordon J, Gantner J, Kemna J, Schwalgun L, Reschke M, Streubel J, Boch J, Stuttmann J
Plant J. 2016 Aug 31. doi: 10.1111/tpj.13319. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 79440 | pDGE1 | recipient plasmid [multiplexing]; p35S:Cas9, nptII |
| 79441 | pDGE2 | recipient plasmid [multiplexing]; p35S:Cas9, BASTA |
| 79442 | pDGE3 | recipient plasmid [multiplexing]; pPcUbi:Cas9, nptII |
| 79443 | pDGE4 | recipient plasmid [multiplexing]; pPcUbi:Cas9, BASTA |
| 79444 | pDGE62 | recipient plasmid [single nuclease]; p35S:Cas9, nptII |
| 79445 | pDGE63 | recipient plasmid [single nuclease]; p35S:Cas9, BASTA |
| 79446 | pDGE64 | recipient plasmid [single nuclease]; pPcUbi:Cas9, nptII |
| 79447 | pDGE65 | recipient plasmid [single nuclease]; pPcUbi:Cas9, BASTA |
| 79448 | pDGE144 | recipient plasmid [multiplexing]; p35S:Cas9, FAST, BASTA |
| 79449 | pDGE146 | recipient plasmid [multiplexing]; pPcUbi:Cas9, FAST, BASTA |
| 79450 | pDGE148 | recipient plasmid [multiplexing]; pDD45:Cas9, FAST, BASTA |
| 79451 | pDGE151 | recipient plasmid [multiplexing]; pICU2:Cas9, FAST, BASTA |
| 79452 | pDGE160 | recipient plasmid [multiplexing]; p35S:Cas9, FAST, nptII |
| 79453 | pDGE161 | recipient plasmid [multiplexing]; pPcUbi:Cas9, FAST, nptII |
| 79454 | pDGE164 | recipient plasmid [multiplexing]; pDD45:Cas9, FAST, nptII |
| 79455 | pDGE165 | recipient plasmid [multiplexing]; pICU2:Cas9, FAST, nptII |
| 79456 | pDGE166 | recipient plasmid [multiplexing]; p35S:Cas9, FAST, Hygro |
| 79457 | pDGE167 | recipient plasmid [multiplexing]; pPcUbi:Cas9, FAST, Hygro |
| 79458 | pDGE168 | recipient plasmid [multiplexing]; pDD45:Cas9, FAST, Hygro |
| 79459 | pDGE169 | recipient plasmid [multiplexing]; pICU2:Cas9, FAST, Hygro |
| 79461 | pDGE5 | sgRNA shuttle vector; module 1 |
| 79462 | pDGE7 | sgRNA shuttle vector; module 2 |
| 79463 | pDGE8 | sgRNA shuttle vector; module 2E |
| 79465 | pDGE9 | sgRNA shuttle vector; module 3 |
| 79466 | pDGE10 | sgRNA shuttle vector; module 4 |
| 79467 | pDGE11 | sgRNA shuttle vector; module 4E |
| 79469 | pDGE12 | sgRNA shuttle vector; module 5 |
| 79471 | pDGE13 | sgRNA shuttle vector; module 6 |
| 79473 | pDGE14 | sgRNA shuttle vector; module 7 |
| 79474 | pDGE15 | sgRNA shuttle vector; module 8E |
| 79476 | pDGE6 | sgRNA shuttle vector; module 1E |
| 80578 | pDGE76 | recipient plasmid [multiplexing, nickase]; p35S:Cas9 D10A, nptII |
| 80579 | pDGE77 | recipient plasmid [multiplexing, nickase]; p35S:Cas9 D10A, BASTA |
| 80580 | pDGE78 | recipient plasmid [multiplexing, nickase]; pPcUbi:Cas9 D10A, nptII |
| 80581 | pDGE79 | recipient plasmid [multiplexing, nickase]; pPcUbi:Cas9 D10A, BASTA |
| 80582 | pJOG250 | recipient plasmid [multiplexing, activator]; p35S:Cas9 D10A/N863A-Hax3CT, nptII |
| 80583 | pJOG251 | recipient plasmid [multiplexing, activator]; p35S:Cas9 D10A/N863A-Hax3CT, BASTA |
| 80584 | pJOG252 | recipient plasmid [single activator]; p35S:Cas9 D10A/N863A-Hax3CT, nptII |
| 80585 | pJOG253 | recipient plasmid [single activator]; p35S:Cas9 D10A/N863A-Hax3CT, BASTA |
| 80586 | pJOG292 | empty vector (lacZ) for 2nd generation recipient assembly |
| 80587 | pJOG294 | dummy module (ccdB) for 2nd generation recipient assembly |