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Plasmid#199180PurposeThis plasmid is used to express a EF-hand disabled human MICU1 protein in E. coli. The protein is fused with a maltose binding protein in the N-terminus, and also contains a C-terminal His6 tag.DepositorInsertMICU1 (MICU1 Human)
UseTagsHis6 and MBP, Thrombin site, TEV siteExpressionMutationD421A, E432K, F433W, F453WPromoterAvailable sinceAug. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
K365-micro
Plasmid#188457PurposeComponent of optically-induced motor dimerization systemDepositorInsertKinesin 1-365 (Khc Fly)
UseTags6xHis-MBP-TEV and SspB microExpressionBacterialMutationKinesin amino acids 1-365PromoterT7Available sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
hTln1-R1R2-17b
Plasmid#191427PurposeExpresses a variant of the human TLN1 R1R2 domains containing a 17AA insert into R2 (exon 17b) in bacteriaDepositorInsertHuman Talin1 R1R2 domains (TLN1 Human)
UseTagsHis-tag, TEV cleavage siteExpressionBacterialMutationcontains 17AA insertion from inclusion of exon 17bPromoterAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a Cdk2ap1CAN-MutTER
Plasmid#178033PurposeExpression vector for the purpose of protein purification.DepositorInsertCdk2ap1 (Cdk2ap1 Mouse)
UseNonviralTags6xHIS - Thrombin - MBP- TEV-TRSExpressionBacterialMutationChanged T(108)A, E(109)A, R(110)APromoterT7LacIAvailable sinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a Cdk2ap1ΔN (MT2B2)
Plasmid#178034PurposeExpression vector for the purpose of protein purification.DepositorInsertCdk2ap1 (Cdk2ap1 Mouse)
UseNonviralTags6xHIS - Thrombin - MBP- TEV-TRSExpressionBacterialMutationFirst N-Terminal 27aa are deletedPromoterT7LacIAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
MSNA-c029
Plasmid#175471PurposeProtein expression in bacterial cells. FERM domain, M1-E346. Contains L281R and H288A point mutations that reduce binding to CD44.DepositorInsertMSN (MSN Human)
UseTagsHis6-TEVExpressionBacterialMutationL281R, H288APromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
SYKA-c022
Plasmid#175490PurposeProtein expression in bacterial cells. Tandem SH2 domains, M6-N269. Can be biotinylated.DepositorInsertSYK (SYK Human)
UseTagsAviTag and His6-TEVExpressionBacterialMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
MSNA-c028
Plasmid#175470PurposeProtein expression in bacterial cells. FERM domain, M1-E346. Contains H288A point mutation that reduces binding to CD44.DepositorInsertMSN (MSN Human)
UseTagsHis6-TEVExpressionBacterialMutationH288APromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
MSNA-c021
Plasmid#175468PurposeProtein expression in bacterial cells. FERM domain, M1-E346. Contains L281R point mutation that reduces binding to CD44.DepositorInsertMSN (MSN Human)
UseTagsHis6-TEVExpressionBacterialMutationL281RPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
SYKA-c020
Plasmid#175489PurposeProtein expression in bacterial cells. Tandem SH2 domains, M6-N269. Can be used for crystallography.DepositorInsertSYK (SYK Human)
UseTagsHis6-TEVExpressionBacterialMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
MSNA-c001
Plasmid#175466PurposeProtein expression in bacterial cells. FERM domain, M1-E346. Used for crystallography (PDB:6TXQ).DepositorInsertMSN (MSN Human)
UseTagsHis6-TEVExpressionBacterialMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
CEA-N domain residues 1-132 (CEACAM5)-pET30b
Plasmid#174693PurposePlasmid expressing CEA-N domainDepositorInsertCEA-N Domain
UseTagsTEV Cleavage Site and hexahistidine (HIS) tagExpressionBacterialMutationnonePromoterT7 PromoterAvailable sinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-His6-K2P4.1A-mCherry-StreptagII
Plasmid#158743PurposeExpresses K2P4.1 isoform A with TEV protease cleavable N- and C-terminal affinity tags.DepositorInsertK2P4.1-A (KCNK4 Human)
UseTagsHis6 and mCherry with Streptag IIExpressionYeastMutationResidues 1-264 with N-linked glycosylation sites …PromoterAOX1Available sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-His6-K2P4.1B-mCherry-StreptagII
Plasmid#158744PurposeExpresses K2P4.1 isoform B with TEV protease cleavable N- and C-terminal affinity tags.DepositorInsertK2P4.1-B (KCNK4 Human)
UseTagsHis6 and mCherry with Streptag IIExpressionYeastMutationResidues 1-290 and N-linked glycosylation sites r…PromoterAOX1Available sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAD100_hIRG1_4-461_pvp008
Plasmid#124875PurposeExpresses hCAD Arg273His mutant in E.coliDepositorInsertcis-aconitate decarboxylase (ACOD1 Human)
UseTagsStrepTag and TEV siteExpressionBacterialMutationmutated Arg273 to His, deleted amino acids 1-3 an…PromoterT7Available sinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLAP-BUBR1-K795A
Plasmid#114061Purposetransfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purificationDepositorInsertBUBR1-K795A (BUB1B Human)
UseTagsYFP-TEV-S- Human LAPExpressionMammalianMutationC2823A and G2826A (siRNA-resistance), BUBR1-K795APromoterAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
AF10_OM-LZ
Plasmid#111753PurposeBacterial expression for structure determinationDepositorInsertMLLT10 (MLLT10 Human)
UseTags6x His - TEVExpressionBacterialMutationContains the OM-LZ region of AF10 (residues 720-7…PromoterT7Available sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
DOT1L-CC3
Plasmid#111754PurposeBacterial expression for structure determinationDepositorInsertDOT1L (DOT1L Human)
UseTags8xHis-Thrombin-SUMO-TEVExpressionBacterialMutationContains coiled coil #3 (residues 613-661)PromoterT7Available sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
DOT1L-CC1
Plasmid#111756PurposeBacterial expression for structure determinationDepositorInsertDOT1L (DOT1L Human)
UseTags8xHis-Thrombin-SUMO-TEVExpressionBacterialMutationcontains coiled coil #1 (residues 506-555)PromoterT7Available sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
DOT1L-CC2
Plasmid#111755PurposeBacterial expression for structure determinationDepositorInsertDOT1L (DOT1L Human)
UseTags8xHis-Thrombin-SUMO-TEVExpressionBacterialMutationContains coiled coil #2 (residues 560-608)PromoterT7Available sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only