pET-hMICU1EF
(Plasmid #199180)

• Purpose: This plasmid is used to express a EF-hand disabled human MICU1 protein in E. coli. The protein is fused with a maltose binding protein in the N-terminus, and also contains a C-terminal His6 tag.
• Depositing Lab: Ming-Feng Tsai
• Publication: Tsai et al Proc Natl Acad Sci U S A. 2023 Apr 18;120(16):e2217665120. doi: 10.1073/pnas.2217665120. Epub 2023 Apr 10.

Backbone

• Vector backbone: pET24

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MICU1
• Species: H. sapiens (human)
• Insert Size (bp): 2450
• Mutation: D421A, E432K, F433W, F453W
• Entrez Gene: MICU1 (a.k.a. CALC, CBARA1, EFHA3, MPXPS, ara CALC)
• Tags / Fusion Proteins:
  • His6 (C terminal on insert)
  • MBP, Thrombin site, TEV site (N terminal on insert)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry