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Plasmid#198289PurposeProduces PVCs retargeted with knob domain from hAd5 virusDepositorInsertPVCpnf_pvc13-Ad5Knob
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
mCherry-EB1-8
Plasmid#55035PurposeLocalization: MT End Binding Protein, Excitation: 587, Emission: 610DepositorInsertEB1 (MAPRE1 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
Bassik lab Human CRISPR-Cas9 Deletion Library - Membrane proteins
Pooled Library#101929PurposeBassik lab Human CRISPR-Cas9 Deletion Library - Membrane proteins. Coexpresses mCherry.DepositorExpressionMammalianUseCRISPRAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-Ace-mNeon2-Kv2.1PR
Plasmid#195522PurposeGreen fluorescent, negative response-polarity voltage indicator under the control of synapsin promoter; soma-targetedDepositorInsertAce-mNeon2-Kv2.1 proximal restriction sequence
UseAAVTagsExpressionMammalianMutationAce-mNeon 81S; SY linkerPromoterSynapsinAvailable sinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-HOT-P2A-Clover-BlastR
Plasmid#138568PurposeUniversal Cleavable Donor Plasmid for tagging with P2A-Clover-BlastR using NHEJDepositorInsertP2A-clover-BlastR
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3-NL63-D14
Plasmid#172666Purposeencodes NL63 spike protein, for pseudovirus productionDepositorInsertHCNV63gp2 (HCNV63gp2 human coronavirus NL63)
UseLentiviral and RetroviralTagsNAExpressionMammalianMutationc-terminal deletion of 14aaPromoterCMVAvailable sinceJan. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAX APP 751
Plasmid#30138DepositorInsertAmyloid Precursor Protein (APP Human)
UseTagsExpressionMammalianMutationwild type splice variant 751PromoterAvailable sinceAug. 15, 2011AvailabilityAcademic Institutions and Nonprofits only -
hCRISPRa-v2, Kinases, Phosphatases, and Drug Targets (h1), top 5 sgRNAs/gene
Pooled Library#83980PurposeHuman CRISPRa Pooled Library tartgeting kinsases, phosphatases and Drug TargetsDepositorAvailable sinceJan. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pXJ220-pAAV-PB3'IR-U6-gRNA-EF1a-GFP-KASH-PB5'IR
Plasmid#213481PurposeAAV piggybac transposon vector, with GFP-KASH reporter and gRNA cloning site. Compatible with 10X 5' gRNA capture technology.DepositorInsertsGFP-KASH
U6-gRNA
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJR101
Plasmid#187241PurposeLentiviral sgRNA vector for Perturb-seq with mU6 sgRNA promoter, CR1 constant region with CS1 capture sequence in stem loop, and UCOE EF1alpha driving PURO-GFP marker expressionDepositorInsertLentiviral sgRNA vector for Perturb-seq with mU6 promoter
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
FUW-tetO-NFIA
Plasmid#141403PurposeInducible Expression of NFIADepositorInsertNFIA (NFIA Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
hBax C3-EGFP
Plasmid#19741DepositorInsertBax (BAX Human)
UseTagsGFPExpressionMammalianMutationnonePromoterAvailable sinceNov. 10, 2008AvailabilityAcademic Institutions and Nonprofits only -
pMuLE_ENTR_12GLI-FLuc_R4-R3
Plasmid#113712PurposeSingle pathway reporter; hedgehog promoter-driven expression of firefly luciferase; MultiSite Gateway entry vector.DepositorInsert12GLI-FLuc
UseMule gateway entry vectorTagsExpressionMammalianMutationPromoterAvailable sinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
RF12
Bacterial Strain#62077PurposeBL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. trpA trpB genes deleted.DepositorBacterial ResistanceNoneAvailable sinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-EGFP (AAV2)
Viral Prep#50465-AAV2PurposeReady-to-use AAV2 particles produced from pAAV-hSyn-EGFP (#50465). In addition to the viral particles, you will also receive purified pAAV-hSyn-EGFP plasmid DNA. hSyn-driven EGFP expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsEGFPAvailable sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDDGFP_LEU2D
Plasmid#58352PurposeUsed for expressing recombinant proteins as a C terminal GFP-His tagged constuct in S.cerevisiaeDepositorTypeEmpty backboneUseTagsGFP-HisExpressionYeastMutationPromoterGAL1Available sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAM5505
Plasmid#132664PurposepRL623-based helper plasmid for conjugations, with RSF1010 mobA geneDepositorInsertMobA
UseTagsExpressionBacterialMutationplease see depositor comments belowPromoterp1,p3Available sinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFH2.22_mCherry-MS2x8
Plasmid#205537PurposeExpress mCherry-MS2x8 (export tag consisting of 8 tandem repeats of MS2 stem-loop aptamer)DepositorInsertmCherry
UseTagsExpressionMammalianMutationWTPromoterCMVAvailable sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
PLJR962
Plasmid#115162PurposeSth1 dCas9 TetR and KanR L5 Int attP for M. smegmatisDepositorInsertsSth1 sgRNA scaffold
Sth1 dCas9
TetR
L5 attP
L5 Int
KanR
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+-Tornado-nLuc
Plasmid#212611PurposeFor expression of a circular nLuc mRNA. To express your gene of interest clone into the BsiWI, SacII sites and add the GCCATGTcTATGTGG 5' of the SacII site.DepositorInsertTornado-CVB3-nLuc
UseLuciferaseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only