-
PurposeAAV-mediated expression of ArchT-tdTomato for neuronal inhibition
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 29778 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backboneAAV with CAG promoter
-
Backbone manufacturerScott Sternson
- Backbone size w/o insert (bp) 6259
-
Vector typeMammalian Expression, AAV ; Adeno-associated virus
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature30°C
-
Growth Strain(s)NEB Stable
-
Growth instructionsUse recombinase-free E. coli (Stbl3, XL-10, Sure2 et.al), grow at 30C
-
Copy numberLow Copy
Gene/Insert
-
Gene/Insert nameArchT-tdTomato
-
Alt namearchaerhodopsin
-
Alt namearchaerhodopsin TP009
-
Alt nameArchT
-
SpeciesH. strain TP009
-
Insert Size (bp)744
-
MutationN/A
- Promoter CAG
-
Tag
/ Fusion Protein
- tdTomato (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer gctctagagcctctgctaacc
- 3′ sequencing primer gcagcgtatccacatagcg (Common Sequencing Primers)
Resource Information
-
Articles Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
PLEASE CONTACT ED BOYDEN ([email protected]) FOR DETAILS ON THIS REAGENT AND FURTHER REAGENTS IN THIS LINE.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pAAV-CAG-ArchT-tdTomato was a gift from Edward Boyden (Addgene plasmid # 29778 ; http://n2t.net/addgene:29778 ; RRID:Addgene_29778) -
For your References section:
A high-light sensitivity optical neural silencer: development and application to optogenetic control of non-human primate cortex. Han X, Chow BY, Zhou H, Klapoetke NC, Chuong A, Rajimehr R, Yang A, Baratta MV, Winkle J, Desimone R, Boyden ES. Front Syst Neurosci. 2011;5:18. Epub 2011 Apr 13. 10.3389/fnsys.2011.00018 PubMed 21811444