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Plasmid#111905PurposeExpress WKKD mutated RNASEH1 in mammalian cell. The combination of three specific mutations (W43A, K59A, K60A) in the binding domain prevents the enzyme from binding to RNA/DNA hybridsDepositorInsertRNASEH1 (RNASEH1 Human)
UseTagsV5ExpressionMammalianMutationChanged D 210 to N, W 43 to A, K 59 to A, K 60 to…PromoterAvailable sinceJuly 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET16b-Trx
Plasmid#36944DepositorInsertThioredoxin 1 (TXN Human)
UseTagsHis tagExpressionBacterialMutationPromoterT7Available sinceMay 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
p0521s
Plasmid#62862PurposeEpisome vector for Phaeodactylum tricornutum (pCC1BAC-based)DepositorTypeEmpty backboneUseSynthetic Biology; Episomal vector for phaeodacty…TagsExpressionMutationPromoterAvailable sinceMarch 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
psgRNA
Plasmid#114005Purposeexpress sgRNA. ColE1, KanDepositorInsertgRNA
UseTagsExpressionMutationPromoterBBa_J23119Available sinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
FLAG-NLRC5
Plasmid#37521DepositorInsertNLRC5 (NLRC5 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceAug. 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEZYmyc-His
Plasmid#18701PurposepcDNA3.1(-)/Myc-His B converted Gateway Destination vector for expressing C-terminal myc-His tagged fusion proteins in mammalian cellsDepositorTypeEmpty backboneUseTagsMyc-HisExpressionMammalianMutationPromoterCMVAvailable sinceJuly 26, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-ERBB4
Plasmid#29527DepositorInsertERBB4 (ERBB4 Human)
UseTagsExpressionMammalianMutationWild TypePromoterAvailable sinceMay 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Neo-Bam APC 1-331
Plasmid#16508DepositorInsertAPC (APC Human)
UseTagsExpressionMammalianMutationCodon 331 truncationPromoterAvailable sinceApril 30, 2008AvailabilityAcademic Institutions and Nonprofits only -
pHR-MCS-MED1long-IDR-YFP-NLS
Plasmid#145276PurposeMammalian Expression of Nuclear MED1 long IDR-YFP with SV40 NLSDepositorInsertMED1-IDR long (MED1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
p11d-tRPA (MSW#111)
Plasmid#208066Purposeexpression of human Replication Protein A in E. coliDepositorUseTagsExpressionBacterialMutationRPA32 has a point mutation Thr88(ACC) -> Ile(A…PromoterAvailable sinceNov. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
Flag-SRm160
Plasmid#11305DepositorInsertSRm160 (SRRM1 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceApril 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pUHD Cdk1 WT HA
Plasmid#27652DepositorInsertCdk1 (CDK1 Human)
UseTagsHA (YDVPDYA)ExpressionMammalianMutationPromoterAvailable sinceMarch 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
SOX17-3'gRNA
Plasmid#210465PurposegRNA targeting SOX17 3' terminal for CRISPR-Cas9-mediated knock-inDepositorInsertSOX17 (SOX17 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAR014 Lenti pEF-dEnAsCas12a-NLS-VPR-3XHA-T2A-BSD
Plasmid#195548PurposedCas12a effector fusion for manipulating transcriptionDepositorArticleInsertLenti pEF-dEnAsCas12a-NLS-VPR-3XHA-T2A-BSD
UseCRISPR and LentiviralTags3XHAExpressionMammalianMutationPromoterpEF1aAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
eSpCas9(1.1)_No_FLAG_AAVS1_T2
Plasmid#79888PurposeExpresses the AAVS1 T2 gRNA in combination with FLAGless eSpCas9(1.1) to target the AAVS1 "safe harbor" locus.DepositorTypeEmpty backboneUseCRISPRTagsUntagged eSpCas9(1.1)ExpressionMammalianMutationPromoterCBhAvailable sinceJuly 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1_V5_TurboID_FLAG_Nt
Plasmid#194072Purpose3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-blastiDepositorTypeEmpty backboneUseLentiviralTagsV5-TurboID-FLAGExpressionMammalianMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJL1-sfGFP
Plasmid#102634PurposeIn vitro expression of sfGFP from the T7 promoterDepositorInsertsfGFP
UseTagsstrep tagExpressionBacterialMutationPromoterT7Available sinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
ER-J-TAG (pGS730)
Plasmid#160038PurposepLV-A4-UCOE-EF1-3xHA-sfGFP-JPH-TMD-IRES-BLAS-2A-SNAP-WPRE-A2DepositorInsert3xHA-sfGFP-JPH-TMD
UseLentiviralTagsExpressionMutationPromoterEF1Available sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-hM4D(Gi)-mCherry
Plasmid#50461PurposeDouble floxed Gi-coupled hM4D DREADD fused with mCherry under the control of EF1a promoterDepositorInserthM4D(Gi)-mCherry (CHRM4 Human)
UseAAVTagsHA and mCherryExpressionMutationSee supplemental documents for DREADD mutationsPromoterEF1aAvailable sinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
GFP-NPM WT
Plasmid#17578DepositorInsertNPM (NPM1 Human)
UseTagsEGFP and FlagExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2008AvailabilityAcademic Institutions and Nonprofits only