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LCV2 cGAS KO

Plasmid

#217444

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting human cGAS

Depositor

Jarrod Johnson

Article

Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

Insert

sgRNA targeting cGAS (CGAS Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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LCV2 STING KO

Plasmid

#217445

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting human STING

Depositor

Jarrod Johnson

Article

Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

Insert

sgRNA targeting STING (STING1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pDGB3_alpha2_4xCBS-mDFR-Rep-RepA-tNOS (GB3625)

Plasmid

#225433

Purpose

Transcriptional unit for copper-regulation of BeYDV Rep/RepA with 4xCBS domain+minimal promoter DFR for induction by copper.

Depositor

Diego Orzaez

Insert

4xCBS:mDFR:Rep/RepA:Tnos

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pNLF1-C_CBFA2T3:1-615

Plasmid

#211070

Purpose

Mammalian expression of full-length human CBFA2T3. C-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

CBFA2T3:M1-R615 (CBFA2T3 Human)

Use

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

not annotated isoform

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_IGH_CRLF2

Plasmid

#205830

Purpose

Express mEGFP-tagged fusion protein, IGH_CRLF2 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

IGH_CRLF2 (IGH, CRLF2 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2 Blast CRLF2

Plasmid

#194090

Purpose

Expression of human CRLF2 gene

Depositor

Martin Carroll

Article

Hurtz et al J Clin Invest. 2020 Jul 1;130(7):3637-36

Insert

CRLF2 (CRLF2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pBetaActin-FKBP-mScarlet-KIFC1motor

Plasmid

#191334

Purpose

Expresses the fluorescently labeled minus-end directed KIFC1 motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

FKBP-mScarlet-KIFC1 (KIFC1 Human)

Use

Tags

Expression

Mammalian

Mutation

motor domain aa 125-673; N593K

Promoter

Available since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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Circular 200,100 with interspersed loops (FANCC)

Plasmid

#180192

Purpose

AAV vector carrying a guide RNA targeting the human FANCC mRNA

Depositor

Prashant Mali

Article

Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

Insert

Circular 200,100 guide RNA with interspersed loops

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Human U6

Available since

May 17, 2022

Availability

Academic Institutions and Nonprofits only

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Circular 200,100 with interspersed loops (CTNNB1)

Plasmid

#180193

Purpose

AAV vector carrying a guide RNA targeting the human CTNNB1 mRNA

Depositor

Prashant Mali

Article

Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

Insert

Circular 200,100 guide RNA with interspersed loops

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Human U6

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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Circular 200,100 with interspersed loops (SMAD4)

Plasmid

#180191

Purpose

AAV vector carrying a guide RNA targeting the human SMAD4 mRNA

Depositor

Prashant Mali

Article

Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

Insert

Circular 200,100 guide RNA with interspersed loops

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Human U6

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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Circular 200,100 with 8 bp bulge loops (GAPDH)

Plasmid

#170121

Purpose

AAV vector carrying a guide RNA targeting the human GAPDH mRNA

Depositor

Prashant Mali

Article

Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

Insert

Circular 200,100 guide RNA with 8bp bulge loops (GAPDH Human)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Human U6

Available since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pUC-dirTopo-AU1-aGFP

Plasmid

#61973

Purpose

A directional cloning vector of PCR-amplified cDNAs for mammalian expression

Depositor

Hiroshi Udo

Inserts

lacZ alpha
EGFP

Use

Tags

Expression

Mammalian

Mutation

It contains silent mutations

Promoter

Available since

Feb. 4, 2015

Availability

Academic Institutions and Nonprofits only

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FUCas9Cherry

Plasmid

#70182

Purpose

Expresses Cas9 with fluorescent Cherry reporter

Depositor

Marco Herold

Article

Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

Insert

humanised S.pyogenes cas9

Use

CRISPR and Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

hUbC

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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HRE-luciferase

Plasmid

#26731

Purpose

Luciferase reporter construct containing three hypoxia response elements (24-mers) from the Pgk-1 gene.

Depositor

Navdeep Chandel

Article

Emerling et al Proc Natl Acad Sci U S A. 2008 Feb 19. 1

Insert

Hypoxia Responsive Elements (3)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 10, 2010

Availability

Academic Institutions and Nonprofits only

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His6_MBP_FXR1P

Plasmid

#198694

Purpose

Expression of human FXR1 protein in E. coli

Depositor

Simpson Joseph

Article

Edwards et al Sci Rep. 2020 Sep 28;10(1):15858. doi: 1

Insert

FXR1P (FXR1 Human)

Use

Tags

MBP

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 18, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-syn-Flpo (AAV Retrograde)

Viral Prep

#174378-AAVrg

Purpose

Ready-to-use AAV Retrograde particles produced from pAAV-syn-Flpo (#174378). In addition to the viral particles, you will also receive purified pAAV-syn-Flpo plasmid DNA. Syn-driven expression of the recombinase FLPo. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

Depositor

Ian Wickersham

Article

Jin et al Nat Neurosci. 2024 Jan 11. doi: 10.1038/

Promoter

Tags

None

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only
pU6a:sgRNA#2

Plasmid

#64246

Purpose

expresses sgRNA under U6a promoter

Depositor

Wenbiao Chen

Article

Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

May 7, 2015

Availability

Academic Institutions and Nonprofits only

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pJM220 (pUC18T-miniTn7T-gm-rhaSR-PrhaBAD)

Plasmid

#110559

Purpose

miniTn7 delivery plasmid with rhaSR-PrhaBAD inducible promoter

Depositor

Joanna Goldberg

Article

Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

Insert

rhaSR-PrhaBAD

Use

Tags

Expression

Bacterial

Mutation

Promoter

rhaSR-PrhaBAD

Available since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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pHR_Gal4UAS_Tbet_T2A_GFP_PGK_mCherry

Plasmid

#85431

Purpose

Lentiviral vector for Gal4 inducible Tbet T2A GFP and constitutive mCherry expression

Depositor

Wendell Lim

Article

Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

Insert

human Tbet

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pU6-Sp-pegRNA-HEK3_CTT_ins

Plasmid

#132778

Purpose

S. pyogenes pegRNA for CTT insertion at the HEK3 site of human cells using prime editing

Depositor

David Liu

Article

Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

Insert

HEK3_CTT_ins pegRNA

Use

Tags

Expression

Mammalian

Mutation

See manuscript

Promoter

U6

Available since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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