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Showing: 3901 - 3920 of 4755 results

  1. LUC2 (L/Sec/deltaP)

    Plasmid
    #112145
    Purpose
    Mammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activity
    Depositor
    Paul Copeland
    Article
    Mehta et al J Biol Chem. 2004 Sep 3;279(36):37852-9.
    Insert
    Luciferase coding region with Cysteine 258 mutated to selenocysteine (U) and deleted AUGA in PHGPx SECIS core (Gpx4 Rat, Photinus pyralis)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Cysteine 258 mutated to selenocysteine (U) and d…
    Promoter
    Available since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112145-plasmid-map-sequence-id-214904

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  2. pAAV-Hb9-LMO3 (sbGLuc-VChR1-EYFP)

    Plasmid
    #114103
    Purpose
    fusion protein of Gaussia luciferase variant slow burn, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein for bioluminescent optogenetics
    Depositor
    Ute Hochgeschwender
    Article
    Petersen et al Front Neurol. 2022 Jan 20;12:792643. doi
    Insert
    sbGLuc-VChR1-EYFP
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    motoneuron-specific Homeobox 9 promoter
    Available since
    Aug. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    114103-plasmid-map-sequence-id-252555

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  4. UCP3 promoter EP1

    Plasmid
    #71743
    Purpose
    Contains 7kb of the murine UCP3 gene encompassing the core promoter, exon 1, intron 1, and part of exon 2; used to quantify promoter activity
    Depositor
    Heinrich Taegtmeyer
    Article
    Harmancey et al J Biol Chem. 2015 Nov 10. pii: jbc.M115.
    Insert
    Uncoupling Protein 3 promoter (Ucp3 Mouse)
    Use
    Tags
    Luciferase
    Expression
    Bacterial
    Mutation
    Contains 7kb of the murine UCP3 gene encompassing…
    Promoter
    UCP3
    Available since
    Jan. 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    71743-plasmid-map-sequence-id-130165

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  5. p28-2iDP-puro

    Plasmid
    #88893
    Purpose
    Dual-intron DMD platform plasmid. Co-expresses DMD platform segment, firefly luciferase, puroR and mCherry. Plasmid carries phiC31 and Bxb1 attB sites.
    Depositor
    Michele Calos
    Article
    Farruggio et al Biotechnol J. 2017 Jan 31. doi: 10.1002/
    Inserts
    Dual-intron DMD platform
    luciferase
    puromycin resistance enzyme
    mCherry
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Truncated version of the dystrophin protein (tran…
    Promoter
    Available since
    Jan. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    88893-plasmid-map-sequence-id-201484

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  6. LUC45 (L/Sec/mutP)

    Plasmid
    #112148
    Purpose
    Mammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activity
    Depositor
    Paul Copeland
    Article
    Mehta et al J Biol Chem. 2004 Sep 3;279(36):37852-9.
    Insert
    Luciferase coding region with Cysteine 258 mutated to selenocysteine (U) and MUTANT AUGA PHGPx SECIS core (Gpx4 Rat, Photinus pyralis)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Cysteine 258 mutated to selenocysteine (U) and SE…
    Promoter
    Available since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112148-plasmid-map-sequence-id-214874

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  7. pET28a-his-Gluc-iLID

    Plasmid
    #172096
    Purpose
    Expresses a fusion protein of Gluc and iLID, which dimerizes with a sspB-tagged protein when the bioluminescent reaction is initiated, in bacteria
    Depositor
    Avi Schroeder
    Article
    Adir et al Nat Commun. 2022 Apr 28;13(1):2328. doi:
    Insert
    Gaussia Luciferase - iLID
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Gluc Mutations - M43L, M110L
    Promoter
    T7
    Available since
    April 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    172096-plasmid-map-sequence-id-338563

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  10. pAR100

    Plasmid
    #180377
    Purpose
    pAR100 allows cloning of TCRα and TCRβ genes using restriction enzymes (AflII, ApaI, and SacII).
    Depositor
    Alec Redwood
    Article
    Sooda et al Commun Biol. 2022 Feb 16;5(1):133. doi:
    Inserts
    NFAT-Luciferase
    hCD8A
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    hEF1-HTLV and minimal promoter and an NFAT respon…
    Available since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    180377-plasmid-map-sequence-id-370772

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  20. pFrt-invCAG-Luc

    Plasmid
    #63577
    Purpose
    Shuttle vector compatible with Flp-mediated (Flp-in) transgene integration that allows for conditional Luciferase expression upon integration. The vector contains an FRT site and two mutant loxP sites
    Depositor
    Ivo Huijbers
    Article
    Huijbers et al Nat Protoc. 2015 Nov;10(11):1755-85. doi
    Insert
    Stop-Lox71-Luciferase-pA-FRT-Lox66-reverseCAG
    Use
    Cre/Lox; Frt/flpe recombination mediated insertion
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    63577-plasmid-map-sequence-id-105728

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Showing: 3901 - 3920 of 4755 results