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PurposePromoterless luc2 donor plasmid for MPRA library construction
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 49352 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC57
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Backbone manufacturerGenscript
- Backbone size w/o insert (bp) 2693
- Total vector size (bp) 4435
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Vector typeLuciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameluc2
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Alt nameFirefly luciferase
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SpeciesSynthetic
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Insert Size (bp)4435
- Promoter None
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer GTAAAACGACGGCCAGTG
- 3′ sequencing primer GGAAACAGCTATGACCATG (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byPromega
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMPRAdonor1 was a gift from Tarjei Mikkelsen (Addgene plasmid # 49352 ; http://n2t.net/addgene:49352 ; RRID:Addgene_49352) -
For your References section:
Massively parallel reporter assays in cultured Mammalian cells. Melnikov A, Zhang X, Rogov P, Wang L, Mikkelsen TS. J Vis Exp. 2014 Aug 17;(90). doi: 10.3791/51719. 10.3791/51719 PubMed 25177895