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Massively parallel reporter assays in cultured Mammalian cells.

Melnikov A, Zhang X, Rogov P, Wang L, Mikkelsen TS
J Vis Exp. 2014 Aug 17;(90). doi: 10.3791/51719. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
49349pMPRA1Basic backbone for transient transfection of Massively Parallel Reporter Assay (MPRA) libraries
49350pMPRA2Backbone for transient transfection of Massively Parallel Reporter Assays (MPRA) libraries, pPGK-H2Kk for physical selection of transfected cells
49352pMPRAdonor1Promoterless luc2 donor plasmid for MPRA library construction
49353pMPRAdonor2minP-luc2 donor plasmid for MPRA library construction
49354pMPRAdonor3pSV40mod-luc2 donor plasmid for MPRA library construction
53538pMPRA1-CMV-CloverFluorescent reporter control for pMPRA1
53539pMPRA2-CMV-CloverFluorescent reporter control for pMPRA2
53540pMPRA3-CMV-CloverFluorescent reporter control for pMPRA3

Antibodies from Article