Massively parallel reporter assays in cultured Mammalian cells.
Melnikov A, Zhang X, Rogov P, Wang L, Mikkelsen TS
J Vis Exp. 2014 Aug 17;(90). doi: 10.3791/51719.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
49349 | pMPRA1 | Basic backbone for transient transfection of Massively Parallel Reporter Assay (MPRA) libraries |
49350 | pMPRA2 | Backbone for transient transfection of Massively Parallel Reporter Assays (MPRA) libraries, pPGK-H2Kk for physical selection of transfected cells |
49352 | pMPRAdonor1 | Promoterless luc2 donor plasmid for MPRA library construction |
49353 | pMPRAdonor2 | minP-luc2 donor plasmid for MPRA library construction |
49354 | pMPRAdonor3 | pSV40mod-luc2 donor plasmid for MPRA library construction |
53538 | pMPRA1-CMV-Clover | Fluorescent reporter control for pMPRA1 |
53539 | pMPRA2-CMV-Clover | Fluorescent reporter control for pMPRA2 |
53540 | pMPRA3-CMV-Clover | Fluorescent reporter control for pMPRA3 |