...codon-optimized Firefly luciferase genes (e.g., luc2), and NanoLuc® Luciferase are available from the ... creation of NanoLuc® Luciferase from a rather dull 19kDa Oplophorus luciferase by Promega’s Advanced ...than our more common luciferases like firefly (FLuc) and Renilla (RLuc) luciferase. This is important not...or firefly luciferase into mouse livers followed by i.v. injection of furimazine or luciferin 24 hours ...coupled luciferase. This strategy was first applied using RLuc8, an engineered Renilla luciferase, to produce...isn’t science better when we share? NanoLuc® Luciferase-based tools are fantastic examples of technologies...bioluminescence to overcome these issues as traditional luciferases emit at near optimal wavelengths (Fluc emits ...

... 560 61 Luciferin ATP No Japanese firefly(Luciola cruciate/lateralis)Italian firefly(Luciola italica... plasmids expressing luciferase Luciferase reporter assay The typical luciferase-containing plasmid is...cloning of firefly luciferase was published in 1985, several studies utilized luciferase as a genetic reporter...Well-established Biological sources of luciferase Although luciferase isolated from the firefly beetle (Photinus...second most commonly used luciferase. Other organisms from which luciferases have been cloned from include...italica)Southern Russian firefly(Luciola mingrelica) 562 64 Luciferin ATP No Jamaican click beetle(Pyrophorus...plagiophthalamus) 544-593 64 Luciferin ATP No Railroad worms(Phrixothrix hirtus) 630 64 Luciferin ATP No Common...

...types of luciferase found in Addgene’s collection are Firefly luciferase (Fluc), Renilla luciferase (Rluc... Plasmid Collections Luciferase Plasmids Luciferase Plasmid Guide You may also like... Fluorescent...Fluorescent Proteins Blog: Luciferase 101 Blog: Technologies Enabled by NanoLuc® Luciferase Blog: Luminescent ...and NanoLuc® Luciferase (Nluc). Luc2 is the second-generation version of Firefly luciferase that has been...mammalian systems. Other luciferases in our collection include Gaussia luciferase (Gluc) and the red and...green Click-beetle luciferases (CBRluc and CBGluc, respectively). Like Firefly luciferase, Click-beetle red...type of luciferase has advantages and disadvantages depending on the application. Firefly luciferase is considerably...

...by Kendall Morgan and updated in 2022 by Lucie Wilson. Lucie is an Addgene co-op from Northeastern University...

... spoke with our Financial Operations Specialist, Lucy Hammer, about why she did not go. She told me, “...absolute favorite response to this question came from Lucy Hammer, our Financial Operations Specialist. She...

...ensure their employees are feeling comfortable.     Lucie Wilson is a rising junior and bioengineering student...

...reduced error rate for long sequence knock-ins by: Lucie Wilson Le Cong’s lab has developed a method using...

...electrocompetent cells (Default: 4 tubes of Endura Duos, Lucigen, 60242-1) Alternatives include Stbl4 cells or other...Micropulser, 0.1 cm ) 20 mL SOC recovery media (Lucigen, 80026-1) 8X LB Agar + Antibiotic 245 mm bioassay...

..., A mutant Renilla luciferase, catalyzes the oxidation of its substrate, luciferin, which releases energy...alternative to the widely used luciferase reporter gene plasmid pGL3 pGL3 luciferase reporter gene plasmids... issues with fluorescent pH sensors, a set of luciferase-fluorophore pH fusion reporters, pRSETb-pHlash...containing Cas9, EGFP, mCherry, iRFP, tdTomato, luciferase, LacZ, puromycin or CreERT2 can be combined with...gene (EGFP, iRFP, IFP1.4, puromycin, neomycin or luciferase) to create a final lentiviral vector containing...et al. Nat Methods. 2011. High-throughput luciferase reporter system for detection of pre-mRNA splicing...Gideon Dreyfuss lab has developed rapid-response luciferase (firefly P. pyralis) reporter plasmids for use...

...Renilla luciferase variant fused to an FP; when supplied with a luciferase substrate, the luciferase transfers...problems of low luminescence By itself, Renilla luciferase has a low quantum yield, and when transiently...Nano-lantern, a fusion of a mutagenized Renilla luciferase (RLuc8) with Venus. This luminescent technology...transfer (BRET). Photons emitted by a Renilla luciferase variant are used to excite the fused fluorescent...need for an excitation light source. Instead, a luciferase substrate like coelenterazine is supplied in ...eNL constructs use the brightest characterized luciferase, NanoLuc, and its substrate furimazine. In addition... luciferase...

...mechanosensor called PILATeS, Pulling-Induced Luciferase Association Tension Sensor (Zhong et al., 2024...sensor STReTCh, the 11-amino acid BiT fragment of luciferase is embedded in the I10 domain of the human titin...tension. When this happens, BiT and the LgBiT luciferase fragment (expressed separately) assemble and ... tension sensor. The LgBiT (cyan) fragment of luciferase binds to the BiT Tag (also cyan) embedded in ...systems. Most excitingly, assembly of the split luciferase is reversible, making PILATeS suitable for live... B., Prakash, M., & Dunn, A. R. (2024). Split Luciferase Molecular Tension Sensors for Bioluminescent ...

...Administration of Substrate: Some luciferase reporters, such as Renilla luciferase (RLuc) or NanoLuc, require...bacterial reporters that use fluorophores and luciferases to track bacteria in real time, but each of these...Blog Learn more about in vivo imaging tools The luciferase reporter gene The green fluorescent protein reporter...vivo imaging with fluorescent proteins Browse luciferase plasmids Find fluorescent protein biosensors ...

... the split-luciferase system, intein-mediated splicing reconstitutes functional luciferase only when fusions...target gene, in this case a reporter gene (e.g. luciferase or GFP), regardless of whether it interacts with... fusions of the two halves of the luciferase protein interact and enable intein splicing (5). Screening... Sato, M., Tachihara, K., Umezawa, Y.l. Split luciferase as an optical probe for detecting protein-protein...

...viral infection), producing firefly luciferase. Firefly luciferase can readily be measured to quantify...single (non-viral) reporter gene (e.g. firefly luciferase or eGFP) flanked by the EBOV-specific gene start...to transcribe the reporter gene (e.g. firefly luciferase). The polymerase recognizes the gene start signal...

...Once we were on the right track, we switched to luciferases to more easily quantify the effect of the different...GUS::tMAS to confirm transformation success. Luciferases are great quantitative reporters and use a substrate...substrate to produce bioluminescence. Luciferases usually have shorter mRNA and protein half-lives then...Often a FP is fused to a protein of interest to elucidate the subcellular localization of your protein of...

...Reporters in Gene On include a mScarlet reporter, luciferase reporter, and neomycin resistance reporter. Image... of different reporters including mScarlet-I, luciferase, antibiotic resistance markers, and other enzymes...fluorescent proteins, antibiotic resistance, and luciferase. References Gaudelli NM, Komor AC, Rees HA,...

...Protein A-Renilla luciferase and the “prey” proteins are tagged with Firefly luciferase. The bait and prey...immunoprecipitated from the cells using the Protein A tag. The luciferase activities can then be measured and normalized... assess interactions. Higher relative firefly luciferase activity in the Co-IP compared of a control Co-IP...

...enter cultured cells and activate expression of a luciferase reporter. CPP was not required for protein entry...cells! After verifying the specificity of their luciferase assay, they concluded that components in the ...

...receptor, which can then activate expression of a luciferase reporter in the nucleus. The Roth lab showed ...lab also assembled a collection of plasmids to elucidate the role of 14 of the 16 non-visual heterotrimeric...

...GBPb-GAL4 (DNA-binding domain); and a UAS-driven luciferase reporter construct. In the absence of GFP, no...create a complete transcription factor, activating luciferase transcription. Tang et al. coined this system...