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Plasmid Kit#1000000117PurposeThese plasmids use a TALE assembly reaction (STAR) method to construct multiple TALEs in a fast and simple manner.DepositorAvailable sinceJuly 13, 2017AvailabilityAcademic Institutions and Nonprofits only
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pLEW-pHluorin2-PTS1
Plasmid#213776PurposeExpresses the fluorescent protein pH sensor pHlorin2 in the glycosomes of Trypanosomal bruceiDepositorInsertpHlorin2
UseTrypanosoma brucei expressionTagsExpressionMutationAppended a 2 glycine linker and a T. brucei perox…PromoterT. brucei rRNA promotorAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSP1D12W/2Y
Plasmid#124275PurposeStudy of protein insertion into nanodisks by means of changes in tryptophan fluorescenceDepositorInsertMembrane scaffold protein
UseTags7xHis-tagExpressionBacterialMutationTwo tryptophans changed to two tyrosinesPromoterT7Available sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBA310
Plasmid#190002PurposeInducible expression vector for Trypanosoma bruceiDepositorTypeEmpty backboneUseExpression vector for trypanosoma bruceiTagsExpressionMutationPromoterAvailable sinceSept. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENTR/hBIG1
Plasmid#226251PurposeEntry plasmid of hBIG1 for Gateway systemDepositorInsertBIG1 (ARFGEF1 Human)
UseEntry vectorTagsExpressionMutationPromoterAvailable sinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR/HA-hBIG2
Plasmid#226252PurposeEntry plasmid of N-terminally HA-tagged hBIG2 for Gateway systemDepositorInsertBIG2 (ARFGEF2 Human)
UseEntry vectorTagsHAExpressionMutationPromoterAvailable sinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-dCry-V5His
Plasmid#136241PurposeProtein expression of D. melanogaster (Dm) cytochrome with V5 and His tagsDepositorInsertDmCry
UseTagsHis and V5ExpressionMammalianMutationPromoterAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCheY Tc delta C
Plasmid#13520DepositorInsertcruzain
UseTagsCheYExpressionBacterialMutationTruncated variant: stop codon after Gly212PromoterAvailable sinceMarch 11, 2009AvailabilityAcademic Institutions and Nonprofits only -
pFast-dCry-V5His
Plasmid#136240PurposeProtein expression of D. melanogaster (Dm) cytochrome with C-terminal V5 and His tagsDepositorInsertDmCry
UseTagsHis and V5ExpressionInsectMutationPromoterAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4-ZfCry4
Plasmid#136242PurposeProtein expression of Zebrafish (Zf) cytochrome 4 with a C terminal FLAG tagDepositorInsertZfCry4
UseTagsFlagExpressionMammalianMutationD196H- Please see depositor comments belowPromoterAvailable sinceJan. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
saposin B^W
Plasmid#161759PurposeSaposin B (Homo Sapien) containing a single mutation, arginine 38 to tryptophanDepositorInsertsaposin B R38_W
UseTagsHis-tagExpressionBacterialMutationchanged Arginine 38 to TryptophanPromoterT7Available sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
p5E-2.3k-fabp2
Plasmid#84927Purpose–2.3kb promoter of zebrafish fabp2/ifabp gene in Gateway 5’ Entry vectorDepositorInsert–2.3kb promoter of zebrafish fabp2/ifabp gene (fabp2 Zebrafish)
UseTagsNOExpressionBacterialMutationPromoterAvailable sinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET-21(+)Tm9D8* TrpB
Plasmid#207517PurposeExpression of Tm9D8* TrpB for the synthesis of 7-Aza-L-Tryptophan (7AW) from 7-Azaindole (7AI) and serineDepositorInsertTryptophan synthase β-subunit
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSF-G1(6/7FTrp)RS
Plasmid#207621PurposetRNA synthetase/tRNA pair for the in vivo incorporation of 6-Fluoro-L-Tryptophan (6FTrp) and 7-Fluoro-L-Tryptophan (7FTrp) into proteins in E. coli in response to the amber (TAG) codonDepositorInserttRNA synthetase
UseTagsExpressionBacterialMutationPromoterT7Available sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSF-G1(4/5FTrp)RS
Plasmid#207620PurposetRNA synthetase/tRNA pair for the in vivo incorporation of 4-Fluoro-L-Tryptophan (4FTrp) and 5-Fluoro-L-Tryptophan (5FTrp) into proteins in E. coli in response to the amber (TAG) codonDepositorInserttRNA synthetase
UseTagsExpressionBacterialMutationPromoterT7Available sinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-MBP-CI2F
Plasmid#191254PurposeExpresses chymotrypsin inhibitor with an N-terminal MBP fusion proteinDepositorInsertChymotrypsin Inhibitor 2
UseTagsMaltose Binding ProteinExpressionBacterialMutationPromoterAvailable sinceOct. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEPQD1CB0808
Plasmid#177082PurposeMoClo Level 1, position 4, transcriptional unit for transient expression of tryptophan decarboxylase (CrTDC) from Catharanthus roseus promoter regions contains 4x attB sites for recruitment fo gal4AD-phiC31DepositorInserttryptophan decarboxylase (CrTDC) from Catharanthus roseus
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD1CB0774
Plasmid#177064PurposeMoClo Level 1, position 1, transcriptional unit for transient expression of tryptophan decarboxylase (CrTDC) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptideDepositorInserttryptophan decarboxylase (CrTDC) from Catharanthus roseus
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD1CB0773
Plasmid#177063PurposeMoClo Level 1, position 1, transcriptional unit for transient expression of tryptophan decarboxylase (CrTDC) from Catharanthus roseus driven by 35S promoterDepositorInserttryptophan decarboxylase (CrTDC) from Catharanthus roseus
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDule-pCNF
Plasmid#85494PurposeMachinery plasmid expressing permissive pCNF synthetase and cognate amber suppressing tRNA. Incorporates azidoPhenylalanine.DepositorInsertpara-cyanophenylalanine Mj synthetase
UseTagsNoneExpressionBacterialMutationY32L L65V F108W Q109M D158G I159PPromoterlpp (constitutive)Available sinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only