...growing collection of plasmids that are available to industry. Learn more about the available plasmids and ... Distribution to Industry Featured Collections Search Plasmids Browse Plasmids Browse...Addgene's Search to find plasmids available to industry scientists while also adding additional filters...of the plasmids that are currently available to industry scientists. ID Plasmid Gene/Insert PI Antibodies...us know what you would like to see available to industry scientists. Please note this does not guarantee... information on pricing, payment, and billing. Industry Material Transfer Agreements Most Addgene orders...documents, your order will be processed and shipped. Industry Distribution Policy A portion of Addgene’s collection...

...Kapa Hifi the reaction still does NOT work, you can try these new primers: EMX1-Forward: CCATCCCCTTCTGTGAATGT...

...modified alleles). For this reason, many laboratories try to enhance HDR by synchronizing the cells, since ...and context-dependent, it might be beneficial to try multiple CRISPR strategies when setting up your experimental.... This limitation is of particular concern when trying to edit a gene using homology directed repair (...identify the genomic sequence for the gene you are trying to target. However, the exact region of the gene...associated with the locus can be identified by mass spectrometry (proteins), RNA-sequencing (RNAs), and NGS (...265-271. PMID: 29431739 Base editing: precision chemistry on the genome and transcriptome of living cells...

...type and the protein of interest. You may need to try a variety of fixation methods to find the best conditions... Protocols Immunocytochemistry Immunocytochemistry You may also like... Antibody Plasmid...Molecular Biology Reference Introduction Immunocytochemistry is a technique that uses antibodies to detect...

...AstraZeneca. Why I chose an industry postdoc I’m currently doing an industry postdoc in neuroscience at...talked to people with industry experience, the more I heard that getting into industry early would only help...to postdocs in both industry and academia. Pros and cons: The academic vs. industry postdoc  Time: Academic..., many believe that industry postdocs, having already been immersed in an industry environment, have a...biotech. In other words, industry postdocs already have the prerequisite “industry experience” that I’ve ...as a requirement on some industry job postings. Also, if you work in industry for any length of time, ...be higher in an industry setting. Scientific community and mentorship: As an industry postdoc, you might...

...faster to perform. Introduction to flow cytometry Flow cytometry allows users to analyze single cells in... cultures.  Interpreting flow cytometry data To interpret flow cytometry data users will typically start...   Tips for flow cytometry success Break up cell clumps Success in a flow cytometry experiment depends...protocols? If this sounds like you, then flow cytometry can help you overcome these challenges and make...power in their observations. However, while flow cytometry provides analysis at the cellular level, it cannot...higher the level of expression. A routine flow cytometry experiment uses 2 or 3 different fluorescent colors...accommodate up to 30 different colors. This lends flow cytometry particularly well to complex experiments looking...

...your video call.  Expect technical difficulties. To try and circumvent this, test out the video conferencing...Congratulations, you’ve just landed an interview for an industry job! You’ve worked hard to earn this opportunity...you feel a little panicked. You’ve never had an industry interview before. What should you do to get ready...recently started working in the pharmaceuticals industry after earning my Master’s in 2016 and then working...between applying for an academic position versus an industry position is the on-site interviewing process. ...groups without having a formal interview process. Industry interviews, however, were more structured. I met...is what I’ve found helpful for my interviews in industry. Prepare stories to tell in your interview The...

...Introduction to Gating in Flow Cytometry Antibodies 101: Flow Cytometry Antibodies 101: Conventional vs...When you are running flow cytometry, you’ll need various controls to help you set up and analyze your...Flow specific controls The three types of flow cytometry controls we’ll discuss are single color, fluorescence...linked on their commercial antibody pages. Flow cytometry can be a complicated and daunting process. Controls... vs. Spectral Flow Cytometry Resources on Addgene.org Addgene's Antibody Guide Browse Addgene's Antibody...

...that your gating strategy will be based upon. We’ll try to keep it short though, promise! FSC and SSC: scattering...When using flow cytometry to analyze your samples, it is necessary to set up a sequence of gates to be...the area under the curve (A).   While flow cytometry can be used for virtually all kinds of experiments...group already have experience with using flow cytometry, or if you have access to a professional flow ...learned the basic principles of gating in flow cytometry. Let’s briefly recapitulate what we’ve covered...resources on the Addgene blog Antibodies 101: Flow Cytometry  Antibodies 101: Reading a Flow Plot Antibodies...Antibodies 101: Conventional vs. Spectral Flow Cytometry...

...these dyes, as this would create difficulty when trying to resolve those populations. PE/Cy7 and BB515,...   Spectral flow cytometry Full spectrum flow cytometry, or “spectral flow” cytometry, on the other hand...panel! Although conventional flow cytometry and spectral flow cytometry are more alike than they are different...which spectral flow cytometry requires special considerations. Conventional flow cytometry During conventional...Flow cytometry is one of the most powerful tools available to immunologists, allowing for the rapid analysis...cell types. In recent years, full spectrum flow cytometry (“spectral flow”) has emerged as a particularly...conventional flow cytometry, light emitted by a fluorophore is guided to a single detector using a series of...

...inducible promoters. Far removed from the bio-industry’s vision of ‘biofactories’, most successes in metabolic... metabolic circuitry...

...your budget? Email [email protected] to try out the JF dyes. Acknowledgements I thank Brett ...Howard Hughes Medical Institute. Chemistry is dead, long live chemistry! The discovery of green fluorescent...biochemists have developed techniques to make labeling chemistry easier and more functional in complex biological... ligand to a peptide tag (Figure 1c). Click chemistry (e.g., transcyclooctene–tetrazine)– Nonnatural...bioorthogonal chemical reactions (i.e., click chemistry) to attach a fluorophore at the specific site ...groups—including mine—have been revisiting the old chemistry of dyes. The first synthetic dye, mauvine, was...net-neutral dye scaffold due to its established chemistry, small size, brightness, and cell permeability...

...What's a lab group in industry? The collaborative nature of working in industry and how delegating can...based in Switzerland. After completing her PhD in Chemistry and Chemical Engineering with Harry Gray at Caltech...Caltech, Crystal moved on to a Postdoc in the chemistry department at MIT. Crystal now works on protein...Crystal’s exciting career path, to gain insight into industry research, and to get advice on how to succeed ...Advice to scientists who are planning on working in industry. The importance of recognizing and leveraging ...adapting to needs as they arise. Tips on applying for industry jobs and deciphering job listings. Mobility between...

...available to industry scientists  While we’ve focused on expanding COVID-19 plasmids to industry scientists...available to industry scientists To make plasmid sharing more open for academic and industry scientists,...available more broadly: to scientists in academia and industry. As a result over 90% of Addgene’s COVID-19 resources... to Addgene’s new approach of working with the industry-based scientific community. Conversations with...to the scientific community - both academic and industry scientists. This partnership complemented each...we have other plasmid collections available to industry? Over the past 8 years, several organizations ...Addgene and would like to make them available to industry, please reach out at [email protected] ...

...time before the experiment. What fixed it was not trying even harder to start earlier, but simply moving...work harder - it's to work differently.  Tools to Try  Checklists Found in surgical suites, air fields,...physical copy on hand as you run your experiments. Try drawing out a protocol as you read it, or putting...you to use cold block systems or buckets of ice. Try finding ways to intentionally set up your bench to...for you to reduce the chances of making an error. Try a variety of things, including (and maybe especially...

... test them all, so how do you know which ones to try?  Read on and we will provide you with some advice...acid, for example in a Western blot. If you were to try and use such an antibody in an assay for a native... will bind to your primary antibody. If you are trying to visualize the precise location of a protein ...primary:secondaries:conjugates complex. If you are trying to visualize a protein in low abundance on a Western..., and other information to make sure you’re not trying to choose between the same antibody sold under ...be variations within an application. Take immunohistochemistry (IHC), for instance. While an antibody may...anti-tag antibody; and immunoprecipitation-mass spectrometry strategies that isolate an antibody’s immune...

...precision in control of gene expression so be sure to try a few different systems with your gene/protein of...

...KB If you have trouble loading this video, please try viewing on YouTube . Abstract The prokaryotic clustered...adherent cells, aspirate media. Add 20 μl of 0.05% trypsin-EDTA to each well with a clone present. Resuspend...Maeda T, Paw BH, Orkin SH. Journal of Biological Chemistry . 2014. PubMed . Correction of a genetic disease...

...your primer length and increase the Tm if you are trying to clone from genomic DNA, a cDNA library, or by...to conduct 2 ligations for each plasmid you are trying to create, with varying ratios of recipient plasmid...colonies on your recipient plasmid alone plate, you can try ligating the recipient plasmid alone in the presence... that your transformation worked. You could also try varying the amount of recipient plasmid to insert...

...you should give similar workshops a try if you’re ever looking to try out a new technique or reagent! ... bit more about high throughput DNA prepping by trying out NEB’s new NEBNext Ultra II FS DNA Library Prep...technology. Then we split into pairs to actually try out the kit itself. This was great because it forced...