...growing collection of plasmids that are available to industry. Learn more about the available plasmids and ... Distribution to Industry Featured Collections Search Plasmids Browse Plasmids Browse...Addgene's Search to find plasmids available to industry scientists while also adding additional filters...of the plasmids that are currently available to industry scientists. ID Plasmid Gene/Insert PI Antibodies...us know what you would like to see available to industry scientists. Please note this does not guarantee... information on pricing, payment, and billing. Industry Material Transfer Agreements Most Addgene orders...documents, your order will be processed and shipped. Industry Distribution Policy A portion of Addgene’s collection...

...Kapa Hifi the reaction still does NOT work, you can try these new primers: EMX1-Forward: CCATCCCCTTCTGTGAATGT...

...modified alleles). For this reason, many laboratories try to enhance HDR by synchronizing the cells, since ...and context-dependent, it might be beneficial to try multiple CRISPR strategies when setting up your experimental.... This limitation is of particular concern when trying to edit a gene using homology directed repair (...identify the genomic sequence for the gene you are trying to target. However, the exact region of the gene...associated with the locus can be identified by mass spectrometry (proteins), RNA-sequencing (RNAs), and NGS (...265-271. PMID: 29431739 Base editing: precision chemistry on the genome and transcriptome of living cells...

...precision in control of gene expression so be sure to try a few different systems with your gene/protein of...

...KB If you have trouble loading this video, please try viewing on YouTube . Abstract The prokaryotic clustered...adherent cells, aspirate media. Add 20 μl of 0.05% trypsin-EDTA to each well with a clone present. Resuspend...Maeda T, Paw BH, Orkin SH. Journal of Biological Chemistry . 2014. PubMed . Correction of a genetic disease...

...select a new antibody using the above process and try again. In some cases, it may be preferable to buy...Includes: Immunofluorescence (IF) Immunohistochemistry (IHC) Immunocytochemistry (ICC) Cell Sorting - used to...immunofluorescence (IF), immunohistochemistry (IHC), and immunocytochemistry (ICC) use labeled antibodies... one protein of interest. Immunohistochemistry (IHC) and immunocytochemistry (ICC) IF can refer to both...both immunohistochemistry and immunocytochemistry when fluorescent signaling molecules (also called dyes...Applications - Cell Sorting Methods Flow cytometry Flow cytometry, used commonly in immunology, allows you...capture, and sort single cells. Includes: Flow Cytometry Read on to learn more about the applications common...

...GAGTCCGAGCAGAAGAAGAA 47508 cut S. pyogenes 23792628 Joung ETC2, TRY, and CPC A. thaliana 71288 cut S. pyogenes 26193878...

...available to industry, in addition to academics and nonprofits, and this is denoted in the Industry column ...Plasmid Distribution to Industry page. The list of plasmids available to industry is continually growing...this novel virus. It is now known that SARS-CoV-2 entry, like that of SARS-CoV and MERS CoV, depends on ...plasmids that have been used for measuring viral entry and activity in neutralization assays and links ...growing. ID Plasmid Description Industry PI Other Coronavirus Plasmids: SARS-CoV, MERS, BAT-CoV Plasmids ID...ID Plasmid Description Industry PI Due to export control regulations, MERS and SARS-CoV materials are ... as ACE2 and TMPRSS2, which are involved in the entry of SARS-CoV and SARS-CoV-2. Find plasmids related...

... Plasmid Description Industry Attachments mEmeralds Plasmid Description Industry Attachments Reds Plasmid...Plasmid Description Industry Attachments Blues Plasmid Description Industry Attachments Cyans Plasmid Description...Description Industry Attachments Oranges Plasmid Description Industry Attachments Greens Plasmid Description...Description Industry Attachments Yellows Plasmid Description Industry Attachments Photoactivatables Plasmid...Plasmid Description Industry Attachments Do you have suggestions for other plasmids that should be added ...

...Cre-ERT2 - Tamoxifen inducible; Gateway entry vector none Entry Vector Zon 27493 pBT140 Cre CMV Mammalian...with stop codon Entry vector Ko 139546 pENTR-CreStopDeleted Cre without stop codon Entry vector Ko 139547...Cre T. brucei Cross 33345 pLEW100cre-EP1 Cre Trypanosoma brucei Cross 34564 pMSCVpuro-Cre Cre 5'LTR Retroviral...components, PhyBdeltaCreC and PIF6CreN, in middle entry vector (Tol2 kit) Zebrafish Eisenhoffer 131783 pTol2...139547 pENTR-CreERT2 Cre-ERT2 with stop codon Entry vector Ko 139670 pTFUbiCre Cre Maize Ubiquitin Plant...

...Plasmid Distribution to Industry page. The list of plasmids available to industry is continually growing...email updates for the newest plasmids available to industry. These plasmids have not been expressed or functionally... recode) ID Plasmid Description Mutations Tags Industry Return to top Other Resources Addgene COVID-19...

...Jul 29;7:12266. David Savage Tryptophan Visualize intracellular tryptophan levels and flux Nanosensor detection...green- and orange-emitting proteins Ratiometric Matryoshka biosensors from a nested cassette of green- and...characterization of dedicated sensors for FLIM, for ratiometry and with high affinity. PLoS One. 2015 Apr 14... Applicable over a Wide Concentration Range. Biochemistry. 2015 Aug 18;54(32):5054-62. Martin Webb Phosphate... detection of an immunoregulatory tryptophan influx/kynurenine efflux cycle. PLoS Biol. 2007 Oct;5(10)... Grx1-roCherry. Redox Biol. 2019 Feb;21:101071. Dmitry Bilan Temperature Genetically encoded ratiometric...

...phosphoserine in >36,000 phosphosites by tandem mass spectrometry (Barber et al, Nat. Biotech. 2018). This phosphosite...human substrates of kinases of interest by mass spectrometry in a technique called serine-oriented library...kinase-library reactions (SERIOHL-KILR, Barber et al, Biochemistry 2018). Iterative Synthetically Phosphorylated..., Jun JW, Lou HJ, Turk BE, Rinehart J. 2018. Biochemistry . 2018 Aug 7;57(31):4717-4725. PubMed Improved...

...Purity Antibodies are initially titered by spectrophotometry using a NanoDrop spectrophotometer. If needed...your lot, please email [email protected] . Immunocytochemistry (ICC) Cells are fixed and labeled with the...is compared to an untransfected control. Immunohistochemistry (IHC) Tissue sections are fixed, permeabilized...

...full list below. ID Plasmid Gene/Insert Mutations Industry Return to Top MJFF Shared Research Tools Labs ...list below. ID Plasmid Description Gene/Insert Industry PI Return to Top...

...currently assess western blot, immunohistochemistry, and immunocytochemistry. Addgene will continue to work...

... public-private partnership of 13 members from industry and academia financed by the Innovative Medicine...Gateway-compatible pDEST vectors. ID Plasmid Gene/Insert Industry PI Pooled Libraries CRISPR Pooled Libraries are...

...receptor for SARS-CoV-2, in a single round of viral entry and infection. This approach allows researchers ...Lentiviral prep . ID Plasmid Description Mutations Industry PI Due to export control regulations, MERS and...

... HTR3 HTR3B 5-hydroxytryptamine (serotonin) receptor 3B 5-HT3B HTR3C 5-hydroxytryptamine (serotonin) receptor... HTN3 histatin 3 HIS2, HTN2, HTN5 HTR3A 5-hydroxytryptamine (serotonin) receptor 3A 5-HT-3, 5-HT3A, 5-... receptor 3, family member C - HTR3D 5-hydroxytryptamine (serotonin) receptor 3 family member D MGC119636...MGC119636, MGC119637 HTR3E 5-hydroxytryptamine (serotonin) receptor 3, family member E 5-HT3c1, MGC120035,...factor receptor superfamily, member 14 (herpesvirus entry mediator) ATAR, HVEA, HVEM, LIGHTR, TR2 TNFRSF17...Open Reading Frames (ORFs) in pDONR-223 Gateway® Entry vectors from the labs of William Hahn and David ...

...Other/Trypanosoma cruzi yes, cut S. pyogenes Neo Docampo tdTomato/pTREX-b 68709 Other/Trypanosoma cruzi...Mammalian BbsI yes, activate S. pyogenes Jaenisch piCRg Entry 58904 Mammalian BbsI yes, cut S. pyogenes Huangfu...