...AstraZeneca. Why I chose an industry postdoc I’m currently doing an industry postdoc in neuroscience at...talked to people with industry experience, the more I heard that getting into industry early would only help...to postdocs in both industry and academia. Pros and cons: The academic vs. industry postdoc  Time: Academic..., many believe that industry postdocs, having already been immersed in an industry environment, have a...biotech. In other words, industry postdocs already have the prerequisite “industry experience” that I’ve ...as a requirement on some industry job postings. Also, if you work in industry for any length of time, ...be higher in an industry setting. Scientific community and mentorship: As an industry postdoc, you might...

...compartment, you may want to try an SDS-containing detergent like RIPA. You can try a few different lysing ...proteins to migrate…so don’t try eliminating one component entirely. If you are trying to transfer proteins of...10–15 V/cm of gel), your gel may be overheating. Try running it in a cold room or putting ice packs in... an issue, which can happen with large proteins, try lowering the voltage and using a longer run time....weird issue and can’t figure out what’s causing it, try making fresh running buffer. Old or improperly made... in different pore sizes for different proteins: try using a 0.2 µm (psi) membrane for small proteins ...voltages.  If your small protein is over-transferring, try using the faster semi-dry transfer, which works better...

...your video call.  Expect technical difficulties. To try and circumvent this, test out the video conferencing...Congratulations, you’ve just landed an interview for an industry job! You’ve worked hard to earn this opportunity...you feel a little panicked. You’ve never had an industry interview before. What should you do to get ready...recently started working in the pharmaceuticals industry after earning my Master’s in 2016 and then working...between applying for an academic position versus an industry position is the on-site interviewing process. ...groups without having a formal interview process. Industry interviews, however, were more structured. I met...is what I’ve found helpful for my interviews in industry. Prepare stories to tell in your interview The...

...faster to perform. Introduction to flow cytometry Flow cytometry allows users to analyze single cells in... cultures.  Interpreting flow cytometry data To interpret flow cytometry data users will typically start...   Tips for flow cytometry success Break up cell clumps Success in a flow cytometry experiment depends...protocols? If this sounds like you, then flow cytometry can help you overcome these challenges and make...power in their observations. However, while flow cytometry provides analysis at the cellular level, it cannot...higher the level of expression. A routine flow cytometry experiment uses 2 or 3 different fluorescent colors...accommodate up to 30 different colors. This lends flow cytometry particularly well to complex experiments looking...

...permeabilization techniques — and so it may be necessary to try multiple techniques. For example, we have found ...interest (Figure 1). It can therefore be helpful to try all three when looking to label an intracellular ...Introduction to Gating in Flow Cytometry Antibodies 101: Flow Cytometry Antibodies 101: Validation Resources...determine selectivity of an antibody for flow cytometry experiments. This will include a discussion of...detected in the intended application — here, flow cytometry — is the desired target, using one or more of ...antibody that has already been validated for flow cytometry through this approach. If you’re looking for a...Science) initiative has now started producing flow cytometry characterization data for selected antibodies,...

...David Baker, who was awarded the Nobel Prize in Chemistry last week alongside Demis Hassabis and John Jumper...

...Introduction to Gating in Flow Cytometry Antibodies 101: Flow Cytometry Antibodies 101: Conventional vs...When you are running flow cytometry, you’ll need various controls to help you set up and analyze your...Flow specific controls The three types of flow cytometry controls we’ll discuss are single color, fluorescence...linked on their commercial antibody pages. Flow cytometry can be a complicated and daunting process. Controls... vs. Spectral Flow Cytometry Resources on Addgene.org Addgene's Antibody Guide Browse Addgene's Antibody...

...these dyes, as this would create difficulty when trying to resolve those populations. PE/Cy7 and BB515,...   Spectral flow cytometry Full spectrum flow cytometry, or “spectral flow” cytometry, on the other hand...panel! Although conventional flow cytometry and spectral flow cytometry are more alike than they are different...which spectral flow cytometry requires special considerations. Conventional flow cytometry During conventional...Flow cytometry is one of the most powerful tools available to immunologists, allowing for the rapid analysis...cell types. In recent years, full spectrum flow cytometry (“spectral flow”) has emerged as a particularly...conventional flow cytometry, light emitted by a fluorophore is guided to a single detector using a series of...

...that your gating strategy will be based upon. We’ll try to keep it short though, promise! FSC and SSC: scattering...When using flow cytometry to analyze your samples, it is necessary to set up a sequence of gates to be...the area under the curve (A).   While flow cytometry can be used for virtually all kinds of experiments...group already have experience with using flow cytometry, or if you have access to a professional flow ...learned the basic principles of gating in flow cytometry. Let’s briefly recapitulate what we’ve covered...resources on the Addgene blog Antibodies 101: Flow Cytometry  Antibodies 101: Reading a Flow Plot Antibodies...Antibodies 101: Conventional vs. Spectral Flow Cytometry...

...cells (visualized via the FITC channel) and you are trying to see what percentage of total live cells are ... biorender.com.   In such cases, instead of trying to quantify based on percentage positive or negative...Flow Cytometry Conventional vs Spectral Flow Cytometry Beyond Surface Labeling in Flow Cytometry Resource...example of a histogram plot for a yes/no flow cytometry readout. Created with biorender.com.   Using...

...your budget? Email [email protected] to try out the JF dyes. Acknowledgements I thank Brett ...Howard Hughes Medical Institute. Chemistry is dead, long live chemistry! The discovery of green fluorescent...biochemists have developed techniques to make labeling chemistry easier and more functional in complex biological... ligand to a peptide tag (Figure 1c). Click chemistry (e.g., transcyclooctene–tetrazine)– Nonnatural...bioorthogonal chemical reactions (i.e., click chemistry) to attach a fluorophore at the specific site ...groups—including mine—have been revisiting the old chemistry of dyes. The first synthetic dye, mauvine, was...net-neutral dye scaffold due to its established chemistry, small size, brightness, and cell permeability...

...inducible promoters. Far removed from the bio-industry’s vision of ‘biofactories’, most successes in metabolic... metabolic circuitry...

...What's a lab group in industry? The collaborative nature of working in industry and how delegating can...based in Switzerland. After completing her PhD in Chemistry and Chemical Engineering with Harry Gray at Caltech...Caltech, Crystal moved on to a Postdoc in the chemistry department at MIT. Crystal now works on protein...Crystal’s exciting career path, to gain insight into industry research, and to get advice on how to succeed ...Advice to scientists who are planning on working in industry. The importance of recognizing and leveraging ...adapting to needs as they arise. Tips on applying for industry jobs and deciphering job listings. Mobility between...

...available to industry scientists  While we’ve focused on expanding COVID-19 plasmids to industry scientists...available to industry scientists To make plasmid sharing more open for academic and industry scientists,...available more broadly: to scientists in academia and industry. As a result over 90% of Addgene’s COVID-19 resources... to Addgene’s new approach of working with the industry-based scientific community. Conversations with...to the scientific community - both academic and industry scientists. This partnership complemented each...we have other plasmid collections available to industry? Over the past 8 years, several organizations ...Addgene and would like to make them available to industry, please reach out at [email protected] ...

...time before the experiment. What fixed it was not trying even harder to start earlier, but simply moving...work harder - it's to work differently.  Tools to Try  Checklists Found in surgical suites, air fields,...physical copy on hand as you run your experiments. Try drawing out a protocol as you read it, or putting...you to use cold block systems or buckets of ice. Try finding ways to intentionally set up your bench to...for you to reduce the chances of making an error. Try a variety of things, including (and maybe especially...

... test them all, so how do you know which ones to try?  Read on and we will provide you with some advice...acid, for example in a Western blot. If you were to try and use such an antibody in an assay for a native... will bind to your primary antibody. If you are trying to visualize the precise location of a protein ...primary:secondaries:conjugates complex. If you are trying to visualize a protein in low abundance on a Western..., and other information to make sure you’re not trying to choose between the same antibody sold under ...be variations within an application. Take immunohistochemistry (IHC), for instance. While an antibody may...anti-tag antibody; and immunoprecipitation-mass spectrometry strategies that isolate an antibody’s immune...

...you should give similar workshops a try if you’re ever looking to try out a new technique or reagent! ... bit more about high throughput DNA prepping by trying out NEB’s new NEBNext Ultra II FS DNA Library Prep...technology. Then we split into pairs to actually try out the kit itself. This was great because it forced...

... a lot of activities (try for a dozen!). The best approach may not be “let’s try to make this STEM activity...faster than it can be officially regulated. I did not try to sugarcoat the potential dangers of people using...not. But seeing some students really get it, and trying to answer some of their great questions, was immensely...difficult-but-rewarding jobs, and an enthusiasm for coming back and trying again next year. There are many reasons why you...resources: Steam Powered Family  Biology and Chemistry activities from Quatr.us Foldscopes Steve Spangler...

...career development. I try to do a really good job in one area at a time and try to say “no” to other activities...your home and family unless you live alone. If you try to do it all alone, it should be obvious that your...step! There is more than one way to do most tasks – try to resist the impulse to control by insisting things...tell them they will miss you, of course they will. Try really hard not to do this. For a family with working...

...cloning, Gibson Assembly, Golden Gate etc.), and try to find the simplest way to do it (i.e. avoid assembling...incubation, and amount of DNA. As a general rule, try to use an excess of insert compared to the backbone... fragments If you don’t get any positive clone, try transforming the ligation mix in different bacterial... you might want to reconsider your approach, and try a different cloning strategy. Once you've identified...