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Probing protein folding using site-specifically encoded unnatural amino acids as FRET donors with tryptophan.

Miyake-Stoner SJ, Miller AM, Hammill JT, Peeler JC, Hess KR, Mehl RA, Brewer SH
Biochemistry. 2009 Jun 30;48(25):5953-62. doi: 10.1021/bi900426d. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
85494pDule-pCNFMachinery plasmid expressing permissive pCNF synthetase and cognate amber suppressing tRNA. Incorporates azidoPhenylalanine.
85495pDule2-pCNFMachinery plasmid expressing permissive pCNF synthetase and cognate amber suppressing tRNA. Incorporates azidoPhenylalanine.

Antibodies from Article