pCheY Tc delta C
(Plasmid
#13520)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 13520 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCheYTc
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namecruzain
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SpeciesTrypanosoma cruzi
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Insert Size (bp)1338
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MutationTruncated variant: stop codon after Gly212
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Tag
/ Fusion Protein
- CheY (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (destroyed during cloning)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The truncated form of cruzain was engineered by insertion of a stop codon in the gene at a site corresponding to auto-proteolysis observed with the native enzyme.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCheY Tc delta C was a gift from James McKerrow (Addgene plasmid # 13520 ; http://n2t.net/addgene:13520 ; RRID:Addgene_13520) -
For your References section:
Production of crystallizable cruzain, the major cysteine protease from Trypanosoma cruzi. Eakin AE, McGrath ME, McKerrow JH, Fletterick RJ, Craik CS. J Biol Chem. 1993 Mar 25. 268(9):6115-8. PubMed 8454586
Map uploaded by the depositor.