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Addgene

pCheY Tc delta C
(Plasmid #13520)

Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 13520 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCheYTc
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cruzain
  • Species
    Trypanosoma cruzi
  • Insert Size (bp)
    1338
  • Mutation
    Truncated variant: stop codon after Gly212
  • Tag / Fusion Protein
    • CheY (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (destroyed during cloning)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer M13R
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The truncated form of cruzain was engineered by insertion of a stop codon in the gene at a site corresponding to auto-proteolysis observed with the native enzyme.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCheY Tc delta C was a gift from James McKerrow (Addgene plasmid # 13520 ; http://n2t.net/addgene:13520 ; RRID:Addgene_13520)
  • For your References section:

    Production of crystallizable cruzain, the major cysteine protease from Trypanosoma cruzi. Eakin AE, McGrath ME, McKerrow JH, Fletterick RJ, Craik CS. J Biol Chem. 1993 Mar 25. 268(9):6115-8. PubMed 8454586